Osa-miR1320 targets the ERF transcription factor OsERF096 to regulate cold tolerance via JA-mediated signaling.
Osa-miR1320 targets the ERF transcription factor OsERF096 to regulate cold tolerance via JA-mediated signaling.
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Osa-miR 1320靶向ERF转录因子OsERF 096，通过JA介导的信号传导调节耐寒性。
DOI：
10.1093/plphys/kiac208
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发表时间：
2022-05
期刊：
影响因子：
7.4
通讯作者：
Mingzhe Sun;Yang Shen;Yü-ping Chen;Yan Wang;Xiaoxi Cai;Junkai Yang;Bowei Jia;Weifeng Dong;Xi Chen;Xiaoli Sun
中科院分区：
文献类型：
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作者：
Mingzhe Sun;Yang Shen;Yü-ping Chen;Yan Wang;Xiaoxi Cai;Junkai Yang;Bowei Jia;Weifeng Dong;Xi Chen;Xiaoli Sun
microRNA在非生物胁迫反应中起着关键作用。水稻（Oryza sativa L.）miR 1320是一种物种特异性的miRNA，有助于miR 168调节的免疫。然而，miR 1320是否参与水稻对非生物胁迫的响应仍然是未知的。在这项研究中，我们阐明了miR 1320前体产生两个成熟的miR 1320，miR 1320 - 3 p和miR 1320 - 5 p，并且它们在冷胁迫下都显示出表达降低。遗传证据表明，miR 1320过表达导致耐冷性增加，而miR 1320敲低降低耐冷性。此外，AP 2/ERF（APETALA 2/ethylene-responsive factor）转录因子OsERF 096通过5 '-RACE和双荧光素酶测定被鉴定为miR 1320的靶点。OsERF 096的表达被miR 1320的过表达和敲低所改变，并且在不同组织和冷胁迫下表现出与miR 1320相反的模式。因此，OsERF 096负调控冷胁迫耐受性。此外，我们认为OsERF 096可以结合GCC和DRE顺式元件，并在细胞核中作为转录激活因子。基于RNA-seq和靶向代谢组学分析，我们发现OsERF 096修饰激素含量和信号通路。最后，表型和RT-qPCR分析表明，MeJA施用恢复了OsERF 096-OE系中的冷敏感表型和JA激活的3个DREB（脱水反应元件结合）基因的表达。综上所述，我们的结果强烈表明miR 1320-OsERF 096模块通过抑制JA介导的冷信号通路来调节冷耐受性。
MicroRNAs play key roles in abiotic stress response. Rice (Oryza sativa L.) miR1320 is a species-specific miRNA that contributes to miR168-regulated immunity. However, it is still unknown whether miR1320 is involved in rice response to abiotic stress. In this study, we illustrated that the miR1320 precursor generated two mature miR1320s, miR1320-3p and miR1320-5p, and they both displayed decreased expression under cold stress. Genetic evidence showed that miR1320 overexpression resulted in increased cold tolerance, while miR1320 knockdown reduced cold tolerance. Furthermore, an AP2/ERF (APETALA2/ethylene-responsive factor) transcription factor OsERF096 was identified as a target of miR1320 via 5'-RACE and dual luciferase assays. OsERF096 expression was altered by miR1320 overexpression and knockdown and exhibited an opposite pattern to that of miR1320 in different tissues and under cold stress. Consistently, OsERF096 negatively regulated cold stress tolerance. Furthermore, we suggested that OsERF096 could bind to the GCC and DRE cis-elements and act as a transcriptional activator in the nucleus. Based on RNA-seq and targeted metabolomics assays, we found that OsERF096 modified hormone content and signaling pathways. Finally, phenotypic and RT-qPCR assays showed that MeJA application recovered the cold-sensitive phenotype and JA-activated expression of three DREB (Dehydration Responsive Element Binding) genes in the OsERF096-OE line. Taken together, our results strongly suggest that the miR1320-OsERF096 module regulates cold tolerance by repressing the JA-mediated cold signaling pathway.