Development of Sphere-Polymer Brush Hierarchical Nanostructure Substrates for Fabricating Microarrays with High Performance
Development of Sphere-Polymer Brush Hierarchical Nanostructure Substrates for Fabricating Microarrays with High Performance
复制标题
开发用于制造高性能微阵列的球聚合物刷分层纳米结构基底
DOI：
10.1021/acsami.7b09505
复制
发表时间：
2017
影响因子：
9.5
通讯作者：
Wang Zhenxin
中科院分区：
文献类型：
--
作者：
Liu Xia;Tian Rongrong;Liu Dianjun;Wang Zhenxin
在这项工作中，一个球聚合物刷分级纳米结构修饰的载玻片已被开发用于制造高性能的微阵列。该基底由载玻片上均匀的160 nm二氧化硅颗粒自组装单层和后涂覆的聚（甲基丙烯酸缩水甘油酯）（PGMA）刷层（称为PGMA@3D（160）基底）组成，其可以提供含有丰富环氧基团的三维（3D）聚合物刷，用于直接固定各种生物分子。作为一个典型的例子，三种单糖（4-氨基苯基β-d-吡喃半乳糖苷，4-氨基苯基β-d-吡喃葡萄糖苷和4-氨基苯基α-d-吡喃甘露糖苷）与两种凝集素（生物素化蓖麻凝集素120和生物素化刀豆蛋白A）的相互作用已被PGMA@3D（160）底物为基础的碳水化合物微阵列评估。碳水化合物微阵列显示出良好的选择性，强的多价相互作用，和低检测限（LOD）在皮摩尔范围内没有任何信号放大。此外，所提出的球-聚合物刷分级纳米结构基底可以容易地扩展到制造用于DNA和蛋白质检测的其他类型的微阵列。PGMA@3D（160）基片微阵列与平面环氧基片上制备的二维微阵列相比，具有更高的反应效率和更低的LOD（至少1个数量级），使其成为生物分析和生物医学应用的有前途的平台。
In this work, a sphere-polymer brush hierarchical nanostructure-modified glass slide has been developed for fabricating high-performance microarrays. The substrate consists of a uniform 160 nm silica particle-self-assembled monolayer on a glass slide with a postcoated poly(glycidyl methacrylate) (PGMA) brush layer (termed PGMA@3D(160) substrate), which can provide three-dimensional (3D) polymer brushes containing abundant epoxy groups for directly immobilizing various biomolecules. As a typical example, the interactions of three monosaccharides (4-aminophenyl β-d-galactopyranoside, 4-aminophenyl β-d-glucopyranoside, and 4-aminophenyl α-d-mannopyranoside) with two lectins (biotinylated ricinus communis agglutinin 120 and biotinylated concanavalin A fromCanavalia ensiformis) have been assessed by PGMA@3D(160) substrate-based carbohydrate microarrays. The carbohydrate microarrays show good selectivity, strong multivalent interaction, and low limit of detection (LOD) in the picomolar range without any signal amplification. Furthermore, the proposed sphere-polymer brush hierarchical nanostructure substrates can be easily extended to fabricate other types of microarrays for DNA and protein detection. PGMA@3D(160) substrate-based microarrays exhibit higher reaction efficiencies and lower LODs (by at least 1 order of magnitude) in comparison to those of two-dimensional microarrays, which are fabricated on planar epoxy substrates, making it a promising platform for bioanalytical and biomedical applications.