Effects of Temperature Treatments on Cytosine-Methylation Profiles of Diploid and Autotetraploid Plants of the Alpine Species Ranunculus kuepferi (Ranunculaceae)
Effects of Temperature Treatments on Cytosine-Methylation Profiles of Diploid and Autotetraploid Plants of the Alpine Species Ranunculus kuepferi (Ranunculaceae)
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DOI：
10.3389/fpls.2020.00435
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发表时间：
2020-04
影响因子：
5.6
通讯作者：
E. Syngelaki;Christoph C. F. Schinkel;Simone Klatt;E. Hörandl
中科院分区：
文献类型：
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作者：
E. Syngelaki;Christoph C. F. Schinkel;Simone Klatt;E. Hörandl
暴露于环境压力可以触发表观遗传变异，这可能会产生几种进化后果。多倍体似乎影响DNA甲基化谱。然而，目前尚不清楚温度应激是否能诱导不同细胞型的甲基化变化，以及在何种程度上处理转变转化为表观遗传反应。研究这些问题的一个合适的模式系统是高山多年生草本植物毛茛（Ranunculuskuepferi）。在气候生长室中，将kuepferi暴露于寒冷（+7 ° C白天/+2 ° C夜晚;霜冻处理-1 °C冷冲击，每周3晚）和温暖（+15° C白天/+10 ° C夜晚）条件下，连续两个开花期，并在第一个开花期后从一种条件转换到另一种条件。甲基化敏感的扩增片段长度多态性标记被应用于这两年，以追踪可能的变化引起的压力处理。甲基化的模式表明，细胞型在其配置文件中显着不同，独立于治疗年。同样，治疗转变对两种细胞型都有影响，导致癫痫明显减少，无论转变的方向如何。AMOVAs揭示了二倍体处理内的变异高于处理间的变异。在四倍体中，内部甲基化位点在处理间的变异高于处理内，这是对温度变化的双向响应，支持温度胁迫影响表观遗传变异的假说。结果表明，温度敏感性的DNA甲基化模式显示了一个高度动态的表型可塑性在R。kuepferi，因为这两种细胞型都对温度变化作出反应。此外，倍性水平，即使没有杂交的影响，表观遗传背景的变化，这可能与DNA甲基化动态在冷驯化过程中有重要影响。
The exposure to environmental stress can trigger epigenetic variation, which may have several evolutionary consequences. Polyploidy seems to affect the DNA methylation profiles. Nevertheless, it abides unclear whether temperature stress can induce methylations changes in different cytotypes and to what extent a treatment shift is translated to an epigenetic response. A suitable model system for studying these questions is Ranunculus kuepferi, an alpine perennial herb. Diploid and autotetraploid individuals of R. kuepferi were exposed to cold (+7°C day/+2°C night; frost treatment −1°C cold shocks for 3 nights per week) and warm (+15° day/+10°C night) conditions in climate growth chambers for two consecutive flowering periods and shifted from one condition to the other after the first flowering period. Methylation-sensitive amplified fragment-length polymorphism markers were applied for both years, to track down possible alterations induced by the stress treatments. Patterns of methylation suggested that cytotypes differed significantly in their profiles, independent from year of treatment. Likewise, the treatment shift had an impact on both cytotypes, resulting in significantly less epiloci, regardless the shift's direction. The AMOVAs revealed higher variation within than among treatments in diploids. In tetraploids, internally-methylated loci had a higher variation among than within treatments, as a response to temperature's change in both directions, and support the hypothesis of temperature stress affecting the epigenetic variation. Results suggest that the temperature-sensitivity of DNA methylation patterns shows a highly dynamic phenotypic plasticity in R. kuepferi, as both cytotypes responded to temperature shifts. Furthermore, ploidy level, even without effects of hybridization, has an important effect on epigenetic background variation, which may be correlated with the DNA methylation dynamics during cold acclimation.