Identification and Characterization of Genes Required for Cell-to-Cell Fusion in Neurospora crassa
Identification and Characterization of Genes Required for Cell-to-Cell Fusion in Neurospora crassa
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DOI：
10.1128/ec.05003-11
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发表时间：
2011-08-01
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影响因子：
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通讯作者：
Free, Stephen J.
中科院分区：
文献类型：
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作者：
Fu, Ci;Iyer, Priyadarshini;Free, Stephen J.
采用筛选方法从粗糙脉孢菌单基因缺失文库中筛选菌丝融合突变体。突变体与24个细胞融合基因的改变需要分生孢子吻合管（CAT）之间的细胞融合进行了鉴定和表征。鉴定的细胞融合基因包括14个可能在细胞融合发生所需的信号转导途径中起作用的基因（mik-1、mek-1、mak-1、nrc-1、mek-2、mak-2、rac-1、pp 2A、so/ham-1、ham-2、ham-3、ham-5、ham-9和mob 3）。筛选实验还鉴定了细胞融合所需的四种转录因子（adv-1、ada-3、rco-1和snf 5）。在筛选过程中，还鉴定了可能参与囊泡运输过程的三种编码蛋白质的基因（amph-1，ham-10，pkr 1），这是细胞融合所需的。通过筛选程序鉴定的三个基因ham-6、ham-7和ham-8编码可能在介导质膜融合事件中起作用的蛋白质。三个假定的信号转导蛋白，三个转录因子，三个假定的囊泡运输蛋白，和三个蛋白质，可能在介导细胞融合没有被确定为细胞融合所需的先前。
A screening procedure was used to identify cell fusion (hyphal anastomosis) mutants in the Neurospora crassa single gene deletion library. Mutants with alterations in 24 cell fusion genes required for cell fusion between conidial anastomosis tubes (CATs) were identified and characterized. The cell fusion genes identified included 14 genes that are likely to function in signal transduction pathways needed for cell fusion to occur (mik-1, mek-1, mak-1, nrc-1, mek-2, mak-2, rac-1, pp2A, so/ham-1, ham-2, ham-3, ham-5, ham-9, and mob3). The screening experiments also identified four transcription factors that are required for cell fusion (adv-1, ada-3, rco-1, and snf5). Three genes encoding proteins likely to be involved in the process of vesicular trafficking were also identified as needed for cell fusion during the screening (amph-1, ham-10, pkr1). Three of the genes identified by the screening procedure, ham-6, ham-7, and ham-8, encode proteins that might function in mediating the plasma membrane fusion event. Three of the putative signal transduction proteins, three of the transcription factors, the three putative vesicular trafficking proteins, and the three proteins that might function in mediating cell fusion had not been identified previously as required for cell fusion.