Up-regulation of cyclin D1 expression in asthma serum-sensitized human airway smooth muscle promotes proliferation via protein kinase Cα
Up-regulation of cyclin D1 expression in asthma serum-sensitized human airway smooth muscle promotes proliferation via protein kinase Cα
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DOI：
10.3109/01902140903290022
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发表时间：
2010-05-01
影响因子：
1.7
通讯作者：
Qiao, Li-Fen
中科院分区：
文献类型：
--
作者：
Du, Chun-Ling;Xu, Yong-Jian;Qiao, Li-Fen
气道平滑肌细胞的异常肥大和增生在慢性哮喘气道重塑中发挥重要作用。作者之前的研究表明，蛋白激酶 C α (PKC α) 参与被动致敏的人气道平滑肌细胞 (HASMC) 的增殖。然而，潜在的机制仍然未知。在这里，作者使用对人特应性哮喘血清被动致敏的 HASMC 作为模型系统，研究了 PKC α 亚型在控制细胞周期蛋白 D1 表达中的可能作用。培养的 HASMC 用来自特应性哮喘患者的血清被动致敏。细胞增殖通过[
Abnormal hypertrophy and hyperplasia of airway smooth muscle cells play an important role in airway remodeling in chronic asthma. The authors' previous studies have indicated that protein kinase C alpha (PKC alpha) is involved in the proliferation of passively sensitized human airway smooth muscle cells (HASMCs). However, the underlying mechanisms remain unknown. Here, the authors examined the possible role of the alpha isoform of PKC in the control of cyclin D1 expression, using HASMCs passively sensitized on human atopic asthmatic serum as a model system. Cultured HASMCs were passively sensitized with serum from atopic asthmatic patients. Cell proliferation was measured by [