Role for zinc(II) in the copper(I) regulated protein CopY
Role for zinc(II) in the copper(I) regulated protein CopY
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DOI：
10.1016/s0162-0134(01)00378-6
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发表时间：
2002-01-15
影响因子：
3.9
通讯作者：
Dameron, CT
中科院分区：
文献类型：
--
作者：
Cobine, PA;Jones, CE;Dameron, CT
尽管铜硫醇簇在铜代谢调节中很重要，但蛋白质中这些簇的形成化学尚不清楚。可以掺入给定分子内的 COD 离子数量及其配位数各不相同。 CopY 是来自海拉肠球菌的阻遏蛋白，它利用硫醇铜簇来调节铜稳态基因。身体的。纯化的天然重组 apoCopY 的生物学测定表明，在掺入 Cu(I) 之前形成 Zn(II)-蛋白对于获得天然 Cu(I)-S 簇是必要的。在该蛋白质中，Zn(II) 很容易被 Cu(I) 取代。具有同源金属结合基序的 CopY 蛋白被用于研究簇形成稳定性。 (C) 2002 Elsevier Science B.V. 保留所有权利。
Despite the importance of copper-thiolate clusters in the regulation of copper metabolism the formation chemistry of these clusters in proteins is not well understood. The number of COD ions that can be incorporated within a given molecule and their coordination number varies. CopY is a repressor protein from Enterococcus hirae which utilises a copper-thiolate cluster in the regulation of the copper homeostasis genes. Physical. biological assays of purified native reconstituted apoCopY suggest that the formation of a Zn(II)-protein prior to Cu(I) incorporation is necessary to achieve the native Cu(I)-S cluster. In this protein the Zn(II) is readily displaced by the Cu(I). CopY proteins with homologous metal binding motifs are being used to investigate cluster formation stabilisation. (C) 2002 Elsevier Science B.V. All rights reserved.