Phylogenetic and computational structural analysis of VP7 gene of group a human rotavirus G1P[8] strains obtained in Sapporo, Japan from 1987-to 2000
Phylogenetic and computational structural analysis of VP7 gene of group a human rotavirus G1P[8] strains obtained in Sapporo, Japan from 1987-to 2000
复制标题
1987-2000年日本札幌获得的a组人轮状病毒G1P[8]株VP7基因的系统发育和计算结构分析
作者：
Nagakoka Y;Tatsumi M;et al
许多研究表明，G1P [8]基因型是全球最流行的轮状病毒株。虽然两种疫苗已获得许可，其价值在许多国家得到证明，但在引入疫苗之前和之后，需要对循环轮状病毒株的遗传进化进行持续监测。对1987 - 2000年在日本札幌分离的所有田间菌株进行G和P分型。对轮状病毒G1P [8]株的VP7基因进行了系统发育分析。将氨基酸取代映射到预测的三维VP7蛋白质图像上。G1P [8]基因型占优势。对113株G1P [8]基因型菌株进行了分析。VP7基因的系统发育研究将这些菌株分为三个谱系。平均估计的替换率为每年每个位点7.25 × 10 − 4个核苷酸替换。一个主要谱系含有在中和结构域中的残基91和212处具有VP7氨基酸取代的突变株。推测它们位于VP7三聚体的亚基间边界或其附近。这表明，札幌最流行的G1 P [8]谱系菌株通过改变VP7的中和结构域获得了一些生存优势。医学病毒学杂志84：832 - 838，2012.© 2012 Wiley Periodicals，Inc.
Many studies indicate that G1P[8] genotypes are the most prevalent rotavirus strains worldwide. Although two vaccines have been licensed and their value proven in many countries, continuous surveillance for genetic evolution of circulating rotavirus strains before and after the introduction of the vaccines is desirable. G and P typing were carried out on all field strains isolated during 1987–2000 in Sapporo, Japan. Phylogenetic analysis for the VP7 gene of rotavirus G1P[8] strains was performed. Amino acid substitutions were mapped on the predicted three‐dimensional VP7 protein image. G1P[8] genotype predominated. One hundred thirteen strains with G1P[8] genotype were analyzed. Phylogenetic studies of the VP7 gene classified these strains into three lineages. The mean estimated substitution rate was 7.25 × 10−4nucleotide substitutions per site per year. One predominant lineage contained the mutant strains which had VP7 amino acid substitutions at residue 91 and 212 that is in the neutralization domains. They were estimated to locate in or near intersubunit boundary of VP7 trimer. It is suggested that the most prevalent G1P[8] lineage strains in Sapporo obtained some survival advantages by changing the neutralization domains of VP7. J. Med. Virol. 84:832–838, 2012. © 2012 Wiley Periodicals, Inc.