Activation of interleukin-6/STAT3 and liver regeneration following transplantation
Activation of interleukin-6/STAT3 and liver regeneration following transplantation
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DOI：
10.1006/jsre.2001.6086
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发表时间：
2001-04-01
影响因子：
2.2
通讯作者：
Olthoff, KM
中科院分区：
文献类型：
--
作者：
Debonera, F;Aldeguer, X;Olthoff, KM
背景每一个获取、储存和移植的肝脏都会经历冷缺血和再灌注损伤。大多数移植物恢复迅速，但有些移植物受到足够的损伤，导致长期器官功能障碍或需要再次移植。肝移植术后冷缺血再灌注（I/R）后早期移植物功能和恢复的分子机制尚未明确。白细胞介素（IL）-6是肝脏内促有丝分裂反应的关键因子，对细胞周期进展和保护免受损伤很重要。潜在转录因子STAT 3的激活依赖于IL-6的释放。在大鼠原位肝移植（奥尔特）模型中研究了IL-6/STAT 3通路和肝细胞再生在冷缺血和再灌注后移植物恢复和细胞周期进展中的作用。在同系模型中进行大鼠原位奥尔特。在肝移植物中检测IL-6、STAT 3激活和靶向即刻早期基因表达的存在、时间进程和程度，
Background Every liver that is procured, stored, and transplanted experiences injury from cold ischemia and reperfusion. Most recover quickly, but some grafts sustain enough injury to result in prolonged organ dysfunction or require retransplantation. The molecular mechanisms involved in early graft function and recovery following cold ischemia and reperfusion (I/R) after liver transplantation have not been well defined. Interleukin (IL)-6 is a critical factor in the mitogenic response within the liver, and is important for cell cycle progression and protection from injury. Activation of the latent transcription factor, STAT3, is dependent on IL-6 release. The role of the IL-6/STAT3 pathway and hepatocellular regeneration in graft recovery and cell cycle progression following cold ischemia and reperfusion was studied in a rat liver transplant orthotopic (OLT) model.Methods. Rat OLT was performed in a syngeneic model. The presence, time course, and magnitude of expression of IL-6, STAT3 activation, and upregulation of target immediate early genes were determined in liver grafts with minimal (