Surveillance for Zika in Mexico: naturally infected mosquitoes in urban and semi-urban areas
Surveillance for Zika in Mexico: naturally infected mosquitoes in urban and semi-urban areas
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DOI：
10.1080/20477724.2019.1706291
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发表时间：
2020-01-02
影响因子：
3.4
通讯作者：
Moreno-Garcia, Miguel
中科院分区：
文献类型：
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作者：
Correa-Morales, Fabian;Gonzalez-Acosta, Cassandra;Moreno-Garcia, Miguel
墨西哥32个州中有29个州报告了寨卡病例。关于哪些蚊子物种可能在自然界中推动寨卡病毒传播/维持的信息必须定期更新。从2017年1月到2018年11月，在城市和半城市地区使用CDC背包式吸引器在室内和室外收集蚊子，有证据表明蚊子传播疾病传播。使用CDC Trioplex实时RT-PCR对3873个蚊子池进行了寨卡病毒感染检测。对于每个收集的物种，估计感染率的最大似然估计值（MLE）。结果显示，492个蚊子池对寨卡病毒RNA呈阳性。大多数阳性池为埃及伊蚊（Stegomyia）（Linnaeus）（54.6%，MLE = 19）（雄性和雌性）和致倦库蚊（Culex）（Say）（19.5%，MLE = 16.8）。首次在Ae.（Georgecraigius）epactius（Dyar and Knab）（MLE = 17.1），Cx.（Melanoconion）erraticus（Dyar和Knab）（MLE =不可估计）、Culiseta（Culiseta）inornata（Williston）（MLE =不可估计）和Cs（Cs.）particeps（亚当斯）（MLE = 369.5）。其他检测到的物种是：Ae。（Stg.）白纹伊蚊Cx. albopictus（Skuse）（MLE = 90.5）;（Cx.）科罗纳托公司(Dyar和Knab）（MLE = 102.8）和Cx.（Cx.）tarsalis（Coquillett）（MLE = 117.2）。然而，我们的结果不允许将这些物种作为ZIKV的载体。例行监测应开始考虑其他蚊子物种的分类光谱的蚊科。
Zika cases have been reported in 29 out of the 32 states of Mexico. Information regarding which mosquito species might be driving Zika virus transmission/maintenance in nature must be regularly updated. From January 2017 to November 2018, mosquitoes were collected indoors and outdoors using the CDC backpack aspirator in urban and semi-urban areas with evidence of mosquito-borne disease transmission. 3873 mosquito pools were tested for Zika infection using the CDC Trioplex real-time RT-PCR. For each collected specie, maximum likelihood estimator of infection rate (MLE) was estimated. Results showed 492 mosquito pools positive for Zika virus RNA. The majority of the positive pools were Aedes (Stegomyia) aegypti (Linnaeus) (54.6%, MLE = 19) (males and females) and Culex (Culex) quinquefasciatus (Say) (19.5%, MLE = 16.8). For the first time, ZIKV infection was detected in Ae. (Georgecraigius) epactius (Dyar and Knab) (MLE = 17.1), Cx. (Melanoconion) erraticus (Dyar and Knab) (MLE = non-estimable), Culiseta (Culiseta) inornata (Williston) (MLE = non estimable), and Cs (Cs.) particeps (Adams) (MLE = 369.5). Other detected species were: Ae. (Stg.) albopictus (Skuse) (MLE = 90.5), Cx. (Cx.) coronator s.l. (Dyar and Knab) (MLE = 102.8) and Cx. (Cx.) tarsalis (Coquillett) (MLE = 117.2). However, our results do not allow for the incrimination of these species as vectors of ZIKV. Routine surveillance should start to consider other mosquito species across the taxonomic spectrum of the Culicidae.