Adolescent development of neuron structure in dentate gyrus granule cells of male Syrian hamsters.
Adolescent development of neuron structure in dentate gyrus granule cells of male Syrian hamsters.
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雄性叙利亚仓鼠齿状回颗粒细胞神经元结构的青春期发育。
DOI：
10.1002/dneu.20675
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发表时间：
2008
影响因子：
3
通讯作者：
Sisk,CherylL
中科院分区：
文献类型：
--
作者：
Zehr,JuliaL;Nichols,LianaR;Schulz,KalynnM;Sisk,CherylL
海马功能，包括空间认知和应激反应，在青春期成熟。此外，海马神经元结构被性腺类固醇激素修饰，此时性腺类固醇激素急剧增加。本研究探讨了青春期齿状回神经元树突复杂性的变化。在青春期前、中期和晚期雄性叙利亚仓鼠（21、35和49日龄）中追踪颗粒细胞层高尔基体浸渍神经元的树突、棘和细胞体。Sholl分析确定了从索马开始以25 μm增量设置的同心球中包含的交叉点数量和总树枝状长度。分别定量用于Sholl分析的神经元子集的近端和远端节段中的棘密度。我们发现，在青春期，齿状回的下叶，而不是上叶的神经元结构发生了变化。较低的，下锥体叶片显示修剪树突接近细胞体和增加在整个青春期的远端树突棘密度。这些数据表明，齿状回神经元在青春期经历了大量的结构重塑，成熟的模式是区域特异性的。此外，树突结构的这些变化改变了颗粒细胞的电生理特性，可能与海马依赖性认知功能（如学习和记忆）的青少年发育以及海马介导的应激反应有关。© 2008 Wiley Periodicals，Inc.开发神经生物学，2008年。
Hippocampal function, including spatial cognition and stress responses, matures during adolescence. In addition, hippocampal neuron structure is modified by gonadal steroid hormones, which increase dramatically at this time. This study investigated pubertal changes in dendritic complexity of dentate gyrus neurons. Dendrites, spines, and cell bodies of Golgi‐impregnated neurons from the granule cell layer were traced in pre‐, mid‐, and late‐pubertal male Syrian hamsters (21, 35, and 49 days of age). Sholl analysis determined the number of intersections and total dendritic length contained in concentric spheres set at 25‐μm increments from the soma. Spine densities were quantified separately in proximal and distal segments of a subset of neurons used for the Sholl analysis. We found that the structure of neurons in the lower, but not upper, blade of the dentate gyrus changed during adolescence. The lower, infrapyramidal blade showed pruning of dendrites close to the cell body and increases in distal dendritic spine densities across adolescence. These data demonstrate that dentate gyrus neurons undergo substantial structural remodeling during adolescence and that patterns of maturation are region specific. Furthermore, these changes in dendrite structure, which alter the electrophysiological properties of granule cells, are likely related to the adolescent development of hippocampal‐dependent cognitive functions such as learning and memory, as well as hippocampus‐mediated stress responsivity. © 2008 Wiley Periodicals, Inc. Develop Neurobiol, 2008.