Charge-state reduction with improved signal intensity of oligonucleotides in electrospray ionization mass spectrometry
Charge-state reduction with improved signal intensity of oligonucleotides in electrospray ionization mass spectrometry
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DOI：
10.1016/1044-0305(96)80516-2
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发表时间：
1996-08-01
影响因子：
3.2
通讯作者：
Smith, RD
中科院分区：
文献类型：
--
作者：
Muddiman, DC;Cheng, XH;Smith, RD
通过向待电喷雾的溶液中添加有机酸和碱，可以将电喷雾电离质谱中形成的寡核苷酸负离子的电荷状态转变为更高的质荷比。乙酸或甲酸与哌啶和咪唑的组合使用有效地降低了电荷态。当注入溶液含有高百分比的乙腈时，信号强度和稳定性大大增强。此外，在80%乙腈中含有咪唑、哌啶和乙酸的混合物不仅降低了电荷态，而且大大抑制了Na加成。研究了几种碱基组成和长度不同的寡核苷酸，对混合物的研究表明光谱复杂性显着降低。
The shift of charge states of oligonucleotide negative ions formed in electrospray ionization mass spectrometry to higher mass-to-charge ratio has been accomplished by addition of organic acids and bases to the solution to be electrosprayed. The use of acetic acid or formic acid combined with piperidine and imidazole effectively reduced charge states. Signal intensity and stability were enhanced greatly when the infused solution contained a high percentage of acetonitrile. In addition, the cocktail that contained imidazole, piperidine, and acetic acid in 80% acetonitrile not only reduced charge states, but also substantially suppressed Na adduction. Several oligonucleotides that varied in base composition and length were investigated, and studies of mixtures showed a significant reduction in spectral complexity.