Evaluation of conventional media for detection of colonization factor antigens of enterotoxigenic Escherichia coli.
Evaluation of conventional media for detection of colonization factor antigens of enterotoxigenic Escherichia coli.
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评价产肠毒素大肠杆菌定植因子抗原检测的常规培养基。
DOI：
10.1128/jcm.31.8.2163-2166.1993
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发表时间：
1993
影响因子：
9.4
通讯作者：
Hoque,AT
中科院分区：
文献类型：
--
作者：
Ghosh,AR;Sen,D;Sack,DA;Hoque,AT
产肠毒素大肠杆菌菌株的定植因子抗原（CFA）I或II进行了测试，CFA的表达时，生长在16个不同的琼脂培养基上使用凝集和凝固与单克隆抗体，甘露糖耐药血凝，和盐聚集试验。当使用CFA琼脂时，从CFA阳性菌株中检测到CFA，并且当使用其他市售培养基（特别是营养琼脂）时也检测到CFA。当使用其他商业培养基如麦康凯琼脂时，未检测到CFA。营养琼脂与单克隆抗体为基础的凝固试剂的使用提供了一个潜在的简单和快速的方法来检测E。表达CFA I或CFA II的大肠杆菌。
Strains of enterotoxigenic Escherichia coli producing either colonization factor antigen (CFA) I or II were tested for expression of CFA when grown on 16 different agar media by using agglutination and coagglutination with monoclonal antibodies, mannose-resistant hemagglutination, and a salt aggregation assay. CFA was detected from the CFA-positive strains when CFA agar was used, and it was also detected when other commercially available media were used, notably nutrient agar. CFA was not detected when other commercial media such as MacConkey agar were used. The use of nutrient agar with monoclonal antibody-based coagglutination reagents offers a potentially simple and rapid method for detecting E. coli which express CFA I or II.