trans-sialidase from Trypanosoma cruzi induces apoptosis in cells from the immune system in vivo
trans-sialidase from Trypanosoma cruzi induces apoptosis in cells from the immune system in vivo
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DOI：
10.1086/315001
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发表时间：
1999-10-01
影响因子：
6.4
通讯作者：
Campetella, O
中科院分区：
文献类型：
--
作者：
Leguizamón, MS;Mocetti, E;Campetella, O
锥虫（Trypanosoma cnrzi）是南美锥虫病的病原体，其表达转唾液酸酶，该酶能够在大分子之间直接转移唾液酸残基。这种酶会脱落，在疾病的急性期可以在血液中检测到。在感染早期观察到免疫系统的细胞组分的免疫应答和细胞凋亡的几种改变。本文分析了血流转唾液酸酶可能参与这些事件。该酶诱导脾脏、胸腺和外周神经节中免疫系统细胞的凋亡。天然的和重组的唾液酸酶都以相似的程度诱导凋亡。当使用无酶活性的重组分子时，未检测到影响。在剂量反应试验中，即使给予一定量的转唾液酸酶，也可观察到细胞凋亡，而这种量在血液中是酶法检测不到的。这些发现强烈提示唾液酸动员在T。cruzi诱导的免疫系统细胞凋亡。
Trypanosoma cnrzi, the etiologic agent of Chagas' disease, expresses trans-sialidase, an enzyme able to direct transfer of sialyl residues among macromolecules. The enzyme is shed and can be detected in blood during the acute phase of the disease. Several alterations of the immune response and apoptosis of cellular components of the immune system are observed early in the infection. The possible involvement of bloodstream trans-sialidase on these events was analyzed here. The enzyme induced apoptosis in cells of the immune system in the spleen, thymus, and peripheral ganglia. Both natural and recombinant ti ans-sialidases induced apoptosis to a similar extent. No effect was detected when enzymatically inactive recombinant molecules were used. In dose-response assays, apoptosis was observed even when an amount of trans-sialidase was administered that was enzymatically undetectable in blood. These findings strongly suggest a role for sialic acid mobilization in T. cruzi-induced apoptosis of immune system cells.