A new criterion to evaluate water vapor interference in protein secondary structural analysis by FTIR spectroscopy.
A new criterion to evaluate water vapor interference in protein secondary structural analysis by FTIR spectroscopy.
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DOI：
10.3390/ijms150610018
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发表时间：
2014-06-04
影响因子：
5.6
通讯作者：
Ma G
中科院分区：
文献类型：
--
作者：
Zou Y;Ma G
在蛋白质二级结构分析的傅里叶变换红外（FTIR）曲线拟合方法中，二阶导数和傅立叶自反褶积（FSD）是两种常用的方法来解决无特征的酰胺I波段的重叠成分峰。然而，这两种技术的可靠性受到大气中无处不在的水蒸气的极大影响。目前有几个标准被用作质量控制，以确保蛋白质吸收光谱受水蒸气干扰的影响可以忽略不计。在本研究中，通过对液态水的二阶导数研究，我们首先认为，由于我们称之为样品吸光度依赖的水蒸气干扰现象，先前建立的标准不能保证对水蒸气干扰的可靠评估。然后，通过对蛋白质和液态水的对比研究，我们发现即使满足既定标准，蛋白质吸收光谱仍然会受到水蒸气干扰的显著影响。最后，我们建议将蛋白质和液态水的二阶导数光谱的比较作为更好地评价水蒸气干扰的新标准，以便对蛋白质酰胺I波段进行更可靠的二阶导数和FSD处理。
Second derivative and Fourier self-deconvolution (FSD) are two commonly used techniques to resolve the overlapped component peaks from the often featureless amide I band in Fourier transform infrared (FTIR) curve-fitting approach for protein secondary structural analysis. Yet, the reliability of these two techniques is greatly affected by the omnipresent water vapor in the atmosphere. Several criteria are currently in use as quality controls to ensure the protein absorption spectrum is negligibly affected by water vapor interference. In this study, through a second derivative study of liquid water, we first argue that the previously established criteria cannot guarantee a reliable evaluation of water vapor interference due to a phenomenon that we refer to as sample’s absorbance-dependent water vapor interference. Then, through a comparative study of protein and liquid water, we show that a protein absorption spectrum can still be significantly affected by water vapor interference even though it satisfies the established criteria. At last, we propose to use the comparison between the second derivative spectra of protein and liquid water as a new criterion to better evaluate water vapor interference for more reliable second derivative and FSD treatments on the protein amide I band.
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影响因子：
15
作者：
Cho, Younhee;Sagle, Laura B.;Iimura, Satoshi;Zhang, Yanjie;Kherb, Jaibir;Chilkoti, Ashutosh;Scholtz, J. Martin;Cremer, Paul S.
通讯作者：
Cremer, Paul S.
DOI：
10.1111/j.1432-1033.1990.tb19354.x
发表时间：
1990-10-24
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
GOORMAGHTIGH, E;CABIAUX, V;RUYSSCHAERT, JM
通讯作者：
RUYSSCHAERT, JM
影响因子：
5.6
作者：
Ostapchenko, Valeriy G.;Sawaya, Michael R.;Baskakov, Ilia V.
通讯作者：
Baskakov, Ilia V.
影响因子：
2.9
作者：
Rahmelow, K;Hubner, W
通讯作者：
Hubner, W
影响因子：
3.3
作者：
Giugliarelli, Alessandra;Sassi, Paola;Nafie, Laurence
通讯作者：
Nafie, Laurence