Early postnatal maternal separation causes alterations in the expression ofβ3-adrenergic receptor in rat adipose tissue suggesting long-term influence on obesity
Early postnatal maternal separation causes alterations in the expression ofβ3-adrenergic receptor in rat adipose tissue suggesting long-term influence on obesity
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产后早期母体分离导致大鼠脂肪组织中β3肾上腺素受体表达的改变，表明对肥胖的长期影响
DOI：
10.1016/j.bbrc.2013.11.005
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Takeuchi Y
中科院分区：
文献类型：
--
作者：
Miki T;Liu JQ;Ohta K;Suzuki S;Kusaka T;Warita K;Yokoyama T;Jamal M;Ueki M;Yakura T;Tamai M;Sumitani K;Hosomi N;Takeuchi Y
本研究调查了产后早期母亲剥夺对以后生活中脂肪组织生物学特征的影响。 Sprague-Dawley 大鼠被分为母体剥夺组 (MD) 或母养对照组 (MRC)。 MD 是通过在出生后 10-15 天内每天将幼鼠与母亲分开 3 小时来实现的。使用实时 RT-PCR 分析测定棕色和白色脂肪组织中线粒体解偶联蛋白 1 (UCP-1)、β3-肾上腺素能受体 (β3-AR) 和抑制素 (PHB) 的 mRNA 水平。 UCP-1通过β3-AR介导，与能量代谢和消耗密切相关。 PHB 在增殖的组织/细胞中高表达。 10 周龄时，MRC 和 MD 大鼠的体重相似。然而，脂肪组织中关键分子的水平发生了显着改变。白色脂肪组织中PHB mRNA的表达量显着升高,而棕色脂肪组织中β3-AR mRNA的表达量显着降低,UCP-1 mRNA的表达量保持不变。鉴于这些分子影响线粒体代谢，我们的研究表明，产后早期的母亲剥夺会影响脂肪组织增殖的命运，可能导致以后的肥胖。
The effects of early postnatal maternal deprivation on the biological characteristics of the adipose tissue later in life were investigated in the present study. Sprague–Dawley rats were classified as either maternal deprivation (MD) or mother-reared control (MRC) groups. MD was achieved by separating the rat pups from their mothers for 3 h each day during the 10–15 postnatal days. mRNA levels of mitochondrial uncoupling protein 1 (UCP-1), β3-adrenergic receptor (β3-AR), and prohibitin (PHB) in the brown and white adipose tissue were determined using real-time RT-PCR analysis. UCP-1, which is mediated through β3-AR, is closely involved in the energy metabolism and expenditure. PHB is highly expressed in the proliferating tissues/cells. At 10 weeks of age, the body weight of the MRC and MD rats was similar. However, the levels of the key molecules in the adipose tissue were substantially altered. There was a significant increase in the expression of PHB mRNA in the white adipose tissue, while the β3-AR mRNA expression decreased significantly, and the UCP-1 mRNA expression remained unchanged in the brown adipose tissue. Given that these molecules influence the mitochondrial metabolism, our study indicates that early postnatal maternal deprivation can influence the fate of adipose tissue proliferation, presumably leading to obesity later in life.