Para-hydrogen induced polarization of amino acids, peptides and deuterium-hydrogen gas
Para-hydrogen induced polarization of amino acids, peptides and deuterium-hydrogen gas
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DOI：
10.1039/c1cp20992b
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发表时间：
2011-01-01
影响因子：
3.3
通讯作者：
Appelt, Stephan
中科院分区：
文献类型：
--
作者：
Gloeggler, Stefan;Mueller, Rafael;Appelt, Stephan
通过可逆交换的信号放大（SABRE）是通过从仲氢不氢化的极化转移超极化底物的方法。在这里，我们证明，这种方法可以应用于hyperplasticizing少量的所有蛋白质氨基酸和一些选定的肽下降到纳摩尔制度，并可以在一个单一的扫描中检测到低磁场下降到0.25 mT（10 kHz的质子频率）。一个突出的特征是，取决于所用催化剂和所研究的氨基酸或肽的化学状态，形成超极化的氢-氘气，其用（1）H和（2）H NMR光谱在B（0）= 3.9 mT（166 kHz质子频率）和3.2 mT（20 kHz氘频率）的低磁场下检测。
Signal Amplification by Reversible-Exchange (SABRE) is a method of hyperpolarizing substrates by polarization transfer from para-hydrogen without hydrogenation. Here, we demonstrate that this method can be applied to hyperpolarize small amounts of all proteinogenic amino acids and some chosen peptides down to the nanomole regime and can be detected in a single scan in low-magnetic fields down to 0.25 mT (10 kHz proton frequency). An outstanding feature is that depending on the chemical state of the used catalyst and the investigated amino acid or peptide, hyperpolarized hydrogen-deuterium gas is formed, which was detected with (1)H and (2)H NMR spectroscopy at low magnetic fields of B(0) = 3.9 mT (166 kHz proton frequency) and 3.2 mT (20 kHz deuterium frequency).