A function for AAMP in Nod2-mediated NF-κB activation
A function for AAMP in Nod2-mediated NF-κB activation
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DOI：
10.1016/j.molimm.2009.04.022
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发表时间：
2009-08-01
影响因子：
3.6
通讯作者：
Kufer, T. A.
中科院分区：
文献类型：
--
作者：
Bielig, H.;Zurek, B.;Kufer, T. A.
在酵母双杂交筛选中，含有WD 40重复序列的血管相关迁移细胞蛋白（AAMP）被鉴定为人核苷酸结合结构域富含亮氨酸重复序列（NLR）家族成员Nod 2的新结合伴侣。来自人类细胞的免疫共沉淀证实了这种相互作用，并揭示了跨越三个WD 40结构域的AAMP的内部肽足以进行这种相互作用。发现AAMP在不同的人类细胞系中普遍表达，并且在上皮细胞中表现出主要的胞质定位。在功能上，使用过表达和siRNA敲低，我们表明AAMP调节HEK 293 T细胞中Nod 2和Nod 1介导的NF-κ B活化。总而言之，我们的数据支持AAMP在调节NLR蛋白Nod 2引发的先天免疫反应中的新功能。(C)2009爱思唯尔有限公司保留所有权利。
The WD40 repeat containing angio-associated migratory cell protein (AAMP) was identified as a new binding partner of the human nucleotide-binding domain, leucine rich repeat containing (NLR) family member Nod2 in a yeast two-hybrid screen. Co-immunoprecipitations from human cells verified this interaction and revealed that an internal peptide of AAMP spanning three WD40 domains was sufficient for this interaction. AAMP was found to be ubiquitously expressed in different human cell-lines and exhibited a predominant cytosolic localization in epithelial cells. Functionally, using overexpression and siRNA knock-down, we showed that AAMP modulates Nod2- and Nod1-mediated NF-kappa B activation in HEK293T cells. Taken together, our data support a new function of AAMP in regulating innate immune responses initiated by the NLR protein Nod2. (C) 2009 Elsevier Ltd. All rights reserved.