Metaproteomics: Much More than Measuring Gene Expression in Microbial Communities
Metaproteomics: Much More than Measuring Gene Expression in Microbial Communities
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DOI：
10.1128/msystems.00115-19
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发表时间：
2019-05-01
期刊：
影响因子：
6.4
通讯作者：
Kleiner, Manuel
中科院分区：
文献类型：
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作者：
Kleiner, Manuel
代谢蛋白质组学是从微生物群落中大规模鉴定和量化蛋白质，从而在分子水平上提供对微生物表型的直接洞察。最初，代谢蛋白质组学主要用于评估微生物群落成员的代谢和生理学。然而，最近发展的元蛋白质组学工具允许量化每个物种的生物量来确定群落结构、群落成员的原位碳源以及群落成员对标记底物的吸收。从这个角度，我简要概述了我们目前可以解决的问题，以及我们和其他人正在开发的基于元蛋白质组的新方法，以解决微生物群落和动植物微生物区系研究中的更多问题。我还强调了一些领域和技术，我预计未来5年及以后将出现发展和潜在的重大突破。
Metaproteomics is the large-scale identification and quantification of proteins from microbial communities and thus provides direct insight into the phenotypes of microorganisms on the molecular level. Initially, metaproteomics was mainly used to assess the "expressed" metabolism and physiology of microbial community members. However, recently developed metaproteomic tools allow quantification of per-species biomass to determine community structure, in situ carbon sources of community members, and the uptake of labeled substrates by community members. In this perspective, I provide a brief overview of the questions that we can currently address, as well as new metaproteomics-based approaches that we and others are developing to address even more questions in the study of microbial communities and plant and animal microbiota. I also highlight some areas and technologies where I anticipate developments and potentially major breakthroughs in the next 5 years and beyond.