Transcription of woodchuck hepatitis virus in the chronically infected liver.
Transcription of woodchuck hepatitis virus in the chronically infected liver.
复制标题
土拨鼠肝炎病毒在慢性感染的肝脏中的转录。
DOI：
10.1002/j.1460-2075.1985.tb03810.x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
M. Buendia
中科院分区：
文献类型：
--
作者：
T. Möröy;J. Etiemble;C. Trépo;P. Tiollais;M. Buendia
用Northern杂交、核酸酶定位和引物延伸分析等方法研究了土拨鼠肝炎病毒(WHV)基因组在慢性感染土拨鼠肝脏中的转录。在支持主动WHV复制的样本中发现了两个长度分别为2.1和3.7kb的主要转录本，以及几个次要转录本。斑点杂交实验表明，2.1kbRNA代表S基因的主要转录本，编码病毒表面抗原。两个转录起始点位于S前区第二个8月的下游，分别位于S基因翻译起始密码子的上游139核苷酸和152核苷酸。3.7kb的转录本，以相同的数量存在，略大于WHV基因组，可能参与所有病毒蛋白的表达。来自RNA图谱的数据有力地表明，该转录本起始于C基因上游约70个核苷酸，编码病毒核心抗原(WHcAg)，并代表WHcAg的信息。它还可能在病毒复制周期中作为反转录的潜在模板。所有WHV特异性转录本都被发现在一个独特的位置上被处理，即位于核心基因内的多聚腺苷信号下游的20个核苷酸。当只能检测到整合形式的WHV DNA时，在土拨鼠肝细胞癌中观察到一组不同的WHV特异性mRNAs。鉴定了两个大小分别为2.3和4.6 kb的RNA物种。没有3.7kb的RNA，这强化了这个转录本对应于前基因组的假设。
The transcription of woodchuck hepatitis virus (WHV) genome was studied in the liver of chronically infected woodchucks by Northern blot, nuclease mapping and primer extension analysis. Two major transcripts, 2.1 and 3.7 kb in length, and several minor transcripts were found in samples which supported active WHV replication. The 2.1‐kb RNA represents the major transcript of the S gene, encoding the viral surface antigen (WHsAg) as demonstrated by blot‐hybridization experiments. Two transcription initiation sites were localized downstream of the second AUG of the pre‐S region, 139 and 152 nucleotides upstream of the translation initiation codon of the S gene. The 3.7‐kb transcript, present in an equal amount, is slightly larger than the WHV genome and could be involved in the expression of all viral proteins. The data derived from RNA mapping strongly suggest that this transcript is initiated approximately 70 nucleotides upstream of the C gene, encoding the viral core antigen (WHcAg), and represents the message for WHcAg. It might also serve in the viral replication cycle as a potential template for reverse transcription. All WHV‐specific transcripts were found to be processed at a unique site, 20 nucleotides downstream of the polyadenylation signal situated within the core gene. A different set of WHV‐specific mRNAs was observed in a woodchuck hepatocellular carcinoma when only integrated forms of WHV DNA could be detected. Two RNA species of 2.3 and 4.6 kb were characterized. The 3.7‐kb RNA was absent, reinforcing the hypothesis that this transcript corresponds to the pre‐genome.