Identification of the grape basic helix-loop-helix transcription factor family and characterization of expression patterns in response to different stresses
Identification of the grape basic helix-loop-helix transcription factor family and characterization of expression patterns in response to different stresses
复制标题
葡萄碱性螺旋-环-螺旋转录因子家族的鉴定及不同胁迫下表达模式的表征
DOI：
10.1007/s10725-019-00485-3
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发表时间：
2019-05-01
影响因子：
4.2
通讯作者：
Wang, Xiping
中科院分区：
文献类型：
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作者：
Gao, Min;Zhu, Yanxun;Wang, Xiping
碱性螺旋-环-螺旋（Basic helix-loop-helix，bHLH）蛋白是植物中最大的转录因子家族之一，其成员已在模式植物如拟南芥（Arabidopsis thaliana）和水稻（Oryza sativa）中鉴定。然而，对木本植物如葡萄（葡萄属vinifera）中bHLH蛋白的进化历史和表达模式知之甚少。在本研究中，我们在葡萄基因组中共鉴定出126个bHLH基因（VvbHLH），通过系统发育分析将其分为24个亚科，包括4种植物的同源基因。属于相同亚家族的VvbHLH基因具有相似的蛋白质基序和外显子/内含子结构，并且同线性分析表明串联和节段性重复事件是VvbHLH家族扩展的主要贡献者。葡萄与A. thaliana推测bHLH成员有共同的祖先。26 VvbHLH基因的表达分析，代表的III和IV亚家族的成员，揭示了配置文件，是组织特异性的，响应，响应非生物和生物胁迫。两者合计，葡萄bHLH TF的全基因组鉴定和表征提供了对其进化历史的见解，并代表了在作物改良和胁迫耐受性的背景下进一步功能表征的资源。
Basic helix-loop-helix (bHLH) proteins comprise one of the largest transcription factors (TFs) families in plants and members have been identified in model plant species, such as Arabidopsis thaliana and Oryza sativa. However, far less is known about the evolutionary history and expression patterns of bHLH proteins in woody species, such as grape (Vitis vinifera). In this study, we identified a total of 126 bHLH genes (VvbHLH) in grape genome, which were classified into 24 subfamilies based on the phylogenetic analysis included homologs from four plant species. The VvbHLH genes belonging to the same subfamilies have similar protein motifs and exon/intron structures and synteny analysis indicated that tandem and segmental duplication events have been major contributors to the expansion of the VvbHLH family. Synteny analysis between grape and A. thaliana suggested that some bHLH members shared a common ancestor. Expression analysis of 26 VvbHLH genes, representing members of the III and IV subfamilies, revealed profiles that were tissue-specific, hormone-responsive, and responsive to abiotic and biotic stresses. Taken together, the genome-wide identification and characterization of the grape bHLH TFs provide insights into their evolutionary history and represents a resource for further functional characterization in the context of crop improvement and stress tolerance.