Evidence of Burkholderia pseudomallei-Specific Immunity in Patient Sera Persistently Nonreactive by the Indirect Hemagglutination Assay
Evidence of Burkholderia pseudomallei-Specific Immunity in Patient Sera Persistently Nonreactive by the Indirect Hemagglutination Assay
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DOI：
10.1128/cvi.00077-11
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发表时间：
2011-08-01
影响因子：
--
通讯作者：
Norton, Robert E.
中科院分区：
文献类型：
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作者：
Harris, Patrick N. A.;Williams, Natasha L.;Norton, Robert E.
间接血凝试验 (IHA) 是最常用的血清学试验，用于确认是否接触过类鼻疽伯克霍尔德杆菌。经培养确诊的疾病患者在就诊时通常呈非反应性 IHA，并且偶尔无法在系列检测中进行血清转化。我们研究了使用源自持续 IHA 无反应性患者血清培养分离株的抗原是否可以提高 IHA 的敏感性。此外，我们还评估了这组患者对一组拟鼻疽伯克氏菌抗原（包括源自其自身培养分离株的抗原）的抗原特异性淋巴细胞反应。 11 名经培养证实患有类鼻疽的患者被确定为持续存在 IHA 无反应性血清。进行了改良的 IHA，使用对恢复期血清进行测试的患者分离源抗原致敏的红细胞。大多数（82%）血清呈阴性（
The indirect hemagglutination assay (IHA) is the most frequently used serological test to confirm exposure to Burkholderia pseudomallei. Patients with culture-confirmed disease often have a nonreactive IHA at presentation and occasionally fail to seroconvert on serial testing. We investigated whether using antigens derived from the cultured isolates of persistently IHA-nonreactive patient sera improved the sensitivity of the IHA. In addition, we assessed the antigen-specific lymphocyte response in this group of patients to a panel of B. pseudomallei antigens, including those derived from their own cultured isolates. Eleven patients with culture-proven melioidosis were identified as having persistently IHA-nonreactive sera. A modified IHA using erythrocytes sensitized with patient isolate-derived antigen tested against convalescent-phase serum was performed. The majority (82%) of sera showed a negative (