Xenogeneic spermatogenesis following transplantation of hamster germ cells to mouse testes
Xenogeneic spermatogenesis following transplantation of hamster germ cells to mouse testes
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DOI：
10.1095/biolreprod60.2.515
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发表时间：
1999-02-01
影响因子：
3.6
通讯作者：
Brinster, RL
中科院分区：
文献类型：
--
作者：
Ogawa, T;Dobrinski, I;Brinster, RL
最近表明,大鼠精子形成的细精管可能发生在移植后免疫缺陷接受者鼠标从供体老鼠的睾丸细胞,在目前的研究中,仓鼠供体睾丸细胞移植到小鼠是否异基因的精子形成结果,的仓鼠在至少1600万年前分化大于鼠标和产生精子,形状明显不同,鼠标,在总共13只受体小鼠的4个独立实验中，在每只小鼠的睾丸中都发现了仓鼠精子的发生。此外，冷冻保存的仓鼠睾丸细胞在受体体内也产生了精子。然而，受体小鼠精管中的仓鼠精细胞和顶体均出现异常。在受体动物的附睾中也发现了仓鼠精子，但这些精子一般缺乏顶体，且头尾分离。因此，小鼠的仓鼠精子发生过程中出现了精子发生缺陷，这可能反映了内源性小鼠支持细胞完全支持更大的、进化上遥远的仓鼠生殖细胞的能力有限。从冷冻的仓鼠细胞中产生的精子现在将这一物种添加到小鼠和大鼠中，其中的精原干细胞也可以冷冻保存。这一发现可以立即应用于许多物种的珍贵动物，因为这些细胞可以被储存起来，直到找到合适的受体或培养技术来扩大干细胞数量。
It was recently demonstrated that rat spermatogenesis can occur in the seminiferous tubules of an immunodeficient recipient mouse after transplantation of testis cells from a donor rat, In the present study, hamster donor testis cells were transplanted to mice to determine whether xenogeneic spermatogenesis would result, The hamster diverged at least 16 million years ago from the mouse and produces spermatozoa that are larger than, and have a shape distinctly different from, those of the mouse, In four separate experiments with a total of 13 recipient mice, hamster spermatogenesis was identified in the testes of each mouse. Approximately 6% of the tubules examined demonstrated xenogeneic spermatogenesis, In addition, cryopreserved hamster testis cells generated spermatogenesis in recipients. However, abnormalities were noted in hamster spermatids and acrosomes in seminiferous tubules of recipient mice. Hamster spermatozoa were also found in the epididymis of recipient animals, but these spermatozoa generally lacked acrosomes, and heads and tails were separated. Thus, defects in spermiogenesis occur in hamster spermatogenesis in the mouse, which may reflect a limited ability of endogenous mouse Sertoli cells to support fully the larger and evolutionarily distant hamster germ cell. The generation of spermatogenesis from frozen hamster cells now adds this species to the mouse and rat, in which spermatogonial stem cells also can be cryopreserved. This finding has immediate application to valuable animals of many species, because the cells could be stored until suitable recipients are identified or culture techniques devised to expand the stem cell population.