LncRNA expression profile reveals the potential role of lncRNAs in gastric carcinogenesis
LncRNA expression profile reveals the potential role of lncRNAs in gastric carcinogenesis
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DOI：
10.3233/cbm-150460
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发表时间：
2015-01-01
影响因子：
3.1
通讯作者：
Hu, Meijie
中科院分区：
文献类型：
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作者：
Gu, Wei;Gao, Tian;Hu, Meijie
胃癌是我国死亡率居第二位的消化系统恶性肿瘤，发病率居第一位。长链非编码RNA（lncRNA）在胃癌发病机制中的作用尚未见报道。本研究旨在鉴定胃癌发生发展过程中异常表达的lncRNA，并探讨其可能的功能。使用微阵列分析在六对样品中鉴定了在胃肿瘤样品和匹配的邻近正常组织之间显示显著差异表达水平的总共1，297个lncRNA和2，037个mRNA。为了进一步研究它们的功能，lncRNA被分为不同的亚组，mRNA被聚类成几个通路。在另一组10例胃癌患者中，通过实时PCR检测进一步证实了4种lncRNA的表达水平：UCA 1、lincRNA-BBOX 1 -2、CR 594506和BC 015134。编码-非编码共表达网络显示，4个验证的lncRNA与26个mRNA相关，这为这些lncRNA在胃癌进展过程中的潜在作用提供了线索。
Gastric cancer ranks the second highest death rate and is the highest morbidity digestive system malignancy in China. Few reports have elucidated the role of long non-coding RNAs (lncRNAs) in the gastric cancer pathogenesis. The present study was aimed to identify aberrantly expressed lncRNAs involved in the progression of gastric cancer and explored their potential functions. A total of 1,297 lncRNAs and 2,037 mRNAs that showed significantly differential expression level between gastric tumor samples and matched adjacent normal tissues were identified in six pairs of samples using microarray assay. To further explore their functions, lncRNAs were classified into different subgroups and mRNAs were clustered into several pathways. The expression level of 4 lncRNAs: UCA1, lincRNA-BBOX1-2, CR594506 and BC015134 were further confirmed in another cohort of 10 gastric patients by real-time PCR assay. A coding-non-coding co-expression network revealed that the four validated lncRNAs were correlated with twenty-six mRNAs which gave clues about the potential roles of these lncRNAs in the process of gastric cancer progression.