ENZYMOLOGICAL DETERMINATION OF FREE CARNITINE CONCENTRATIONS IN RAT TISSUES.
ENZYMOLOGICAL DETERMINATION OF FREE CARNITINE CONCENTRATIONS IN RAT TISSUES.
复制标题
大鼠组织中游离肉碱浓度的酶学测定。
作者：
N. Marquis;I. Fritz
在已知过量乙酰辅酶A存在的情况下，在耦合分光光度分析中同时测定释放的游离辅酶A。CoA的形成被证明与最初存在的肉碱直接相关。用这种方法分析大鼠组织提取物，发现(-)-肉碱每克干重对心脏、棕色脂肪组织、骨骼肌、睾丸、肾脏、脑和血清的Rmole分别为4.87、3.14、2.68、1.74、1.26、0.55和0.051。描述了测定的特异度和准确性。
In the presence of a known excess of acetyl CoA, free CoA released was determined simultaneously in a coupled spectrophotometric assay. CoA formed was shown to be directly related to carnitine initially present. Rat tissue extracts were analyzed using this method, and rmoles of (-)-carnitine per gram dry weight were found to be 4.87 for heart; 3.14 for brown adipose tissue; 2.68 for skeletal muscle; 1.74 for testes; 1.26 for kidney; 0.55 for brain; and 0.051 for serum. The specificity and accuracy of the determinations are described.