Integrated multi-biosensors based on an ion-sensitive field-effect transistor using photolithographic techniques
Integrated multi-biosensors based on an ion-sensitive field-effect transistor using photolithographic techniques
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DOI：
10.1109/16.30936
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发表时间：
1989-07
影响因子：
3.1
通讯作者：
Y. Hanazato;M. Nakako;S. Shiono;M. Maeda
中科院分区：
文献类型：
--
作者：
Y. Hanazato;M. Nakako;S. Shiono;M. Maeda
利用半导体工艺制备了两种多功能生物传感器，一种对葡萄糖和甘油三油酸酯敏感，另一种对葡萄糖和尿素敏感。一个集成的ISFET（离子敏感场效应晶体管）与三个氢离子敏感的FET元件在一个芯片上被用作传感器的传感器。水溶性的光交联聚合物的沉积技术，使图案化的酶膜（葡萄糖氧化酶，脂肪酶和尿素酶膜）和牛血清白蛋白膜周围的ISFET元件的每个栅极表面的沉积成为可能。用于测量葡萄糖和三油酸甘油酯浓度的多生物传感器同时测定高达5 mM的葡萄糖浓度和高达3 mM的三油酸甘油酯浓度。用于葡萄糖和尿素的生物传感器对于葡萄糖具有0.03至3mM的检测范围，对于尿素具有0.1至20mM的检测范围。一些多生物传感器由于酶污染而表现出交叉敏感性问题。本文介绍了一种防止酶污染的膜制备方法。>
Two kinds of multifunctional biosensors, one sensitive to glucose and triolein and the other to glucose and urea, have been constructed using semiconductor fabrication techniques. An integrated ISFET (ion-sensitive field-effect transistor) with three hydrogen-ion-sensitive FET elements on one chip was used as a transducer for the biosensor. A photolithographic technique with a water-soluble photocrosslinkable polymer made possible the deposition of patterned enzyme membranes (glucose oxidase, lipase, and urease membranes) and bovine serum albumin membrane around each gate surface of ISFET elements. The multibiosensor for measuring glucose and triolein concentrations determined both glucose concentrations up to 5 mM and triolein concentrations up to 3 mM simultaneously. The biosensor for glucose and urea has a detection range of 0.03 to 3 mM for glucose and 0.1 to 20 mM for urea. Some multibiosensors showed a cross-sensitivity problem due to enzyme contamination. An improved membrane fabrication method to prevent the enzyme contamination is described. >