The Drosophila gene abnormal spindle encodes a novel microtubule-associated protein that associates with the polar regions of the mitotic spindle
The Drosophila gene abnormal spindle encodes a novel microtubule-associated protein that associates with the polar regions of the mitotic spindle
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DOI：
10.1083/jcb.137.4.881
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发表时间：
1997-05-19
影响因子：
7.8
通讯作者：
Glover, DM
中科院分区：
文献类型：
--
作者：
Saunders, RDC;Avides, MD;Glover, DM
异常纺锤体是果蝇正常纺锤体结构和功能所需的基因，紧邻基因 tolloid 96A/B。它编码 220 kD 的多肽，预测等电点为 10.8。隐性突变等位基因 asp(1) 指导合成类似于 124 kD 的 COOH 末端截短或内部删除的肽，野生型 Asp 蛋白与微管共纯化，并且不会被已知解离大多数其他微管相关蛋白的盐浓度释放。细菌表达的 NH2 末端 512 个氨基酸肽，具有许多 p34(cdc2) 和 MAP 激酶的潜在磷酸化位点，与微管强烈结合。该分子的中心 579 个氨基酸片段包含一个与许多肌动蛋白捆绑蛋白序列同源的短基序，以及存在于几种蛋白的钙调蛋白结合位点的第二个基序。免疫荧光研究表明，野生型 Asp 蛋白定位于极性紧邻中心体的 thr 纺锤体区域。这些发现与 asp 突变体中已知的纺锤体异常相关进行讨论。
abnormal spindle, a gene required for normal spindle structure and function in Drosophila melanogaster, lies immediately adjacent the gene tolloid at 96A/B. It encodes a 220-kD polypeptide with a predicted pI of 10.8. The recessive mutant allele asp(1) directs the synthesis of a COOH terminally truncated or internally deleted peptide of similar to 124 kD, Wild-type Asp protein copurifies with microtubules and is not released by salt concentrations known to dissociate most other microtubule-associated proteins. The bacterially expressed NH2-terminal 512-amino acid peptide, which has a number of potential phosphorylation sites for p34(cdc2) and MAP kinases, strongly binds to microtubules, The central 579-amino acid segment of the molecule contains one short motif homologous to sequences a number of actin bundling proteins and a second motif present at the calmodulin binding sites of several proteins, Immunofluorescence studies show that the wild-type Asp protein is localized to the polar regions of thr spindle immediately surrounding the centrosome. These findings are discussed in relation to the known spindle abnormalities in asp mutants.