PATHOLOGY OF EXPERIMENTAL AUTOIMMUNE UVEORETINITIS IN MICE
PATHOLOGY OF EXPERIMENTAL AUTOIMMUNE UVEORETINITIS IN MICE
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DOI：
10.1016/0896-8411(90)90144-h
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发表时间：
1990-06-01
影响因子：
12.8
通讯作者：
NUSSENBLATT, RB
中科院分区：
文献类型：
--
作者：
CHAN, CC;CASPI, RR;NUSSENBLATT, RB
本文研究了感光细胞间维甲酸结合蛋白（IRBP）主动免疫小鼠后的实验性自身免疫性葡萄膜视网膜炎（EAU）的组织病理学和免疫病理学。所用方法包括常规光学显微镜和免疫过氧化物酶染色。病变主要位于葡萄膜和视网膜中，并且是特征性的局灶性病变。视网膜的主要组织病理学发现为血管炎、肉芽肿、视网膜皱褶、浆液性脱离和感光细胞丧失。肉芽肿、Dalen-Fuchs结节形成、炎性细胞浸润以及脉络膜和睫状体厚度增加是常见的发现。10%的实验动物发生视网膜下新生血管。在玻璃体中还观察到轻度至中度炎症。视网膜、葡萄膜肉芽肿和Dalen-Fuchs结节中的主要浸润细胞为巨噬细胞。相反，玻璃体中主要的浸润细胞类型是T辅助/诱导淋巴细胞。T抑制细胞/细胞毒性细胞很少见到。眼细胞上Ia抗原的表达仅限于炎症部位的直接区域。免疫后第5周和第10周出现两个病理学高峰，提示该病有复发过程。
The histopathology and immunopathology of murine experimental autoimmune uveoretinitis (EAU) following active immunization with the interphotoreceptor retinoid-binding protein (IRBP) were studied. The methods used included conventional light microscopy and immunoperoxidase staining. Lesions were located mainly in the uvea and the retina and were characteristically focal. The prominent histopathologic findings in the retina were vasculitis, granuloma, retinal fold, serous detachment, and loss of photoreceptors. Granulomas, formation of Dalen-Fuchs nodules, inflammatory cellular infiltration and increase in the thickness of the choroid and ciliary body were frequent findings. Subretinal neovascularization occurred in 10% of the experimental animals. Mild to moderate inflammation was also noted in the vitreous. The predominant infiltrating cells in the retinal and uveal granuloma and Dalen-Fuchs nodules were macrophages. In contrast, the predominant infiltrating cell types in the vitreous were T helper/inducer lymphocytes. T suppressor/cytotoxic cells were rarely seen. Expression of Ia antigens on the ocular cells was confined to the immediate area of the inflammatory sites. The kinetics of histopathology showed two peaks at the 5th and 10th week after immunization, suggesting a relapsing course of the disease.