Mapping the N-linked glycosites of rice (Oryza sativa L.) germinating embryos.
Mapping the N-linked glycosites of rice (Oryza sativa L.) germinating embryos.
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绘制水稻 (Oryza sativa L.) 萌芽胚的 N 连接糖位点图谱
DOI：
10.1371/journal.pone.0173853
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Ying J;Zhao J;Hou Y;Wang Y;Qiu J;Li Z;Tong X;Shi Z;Zhu J;Zhang J
萌发是被子植物生活史中的关键事件。蛋白质的N-糖基化是最常见的翻译后修饰之一，被认为是萌发胚胎蛋白质组的重要调节因子。在这里，我们报道了首次利用亲水相互作用层析(HILIC)糖肽富集法结合高精度质谱学鉴定水稻胚胎萌发过程中的N-连锁糖位。从191个独特的蛋白质中发现了242个糖位。对所涉及的基序和序列结构的检测表明，所有的糖基都集中在[NxS/T]基序中，而82.3%的糖基是卷曲结构。N-糖基化优先发生在糖苷水解酶活性较高的蛋白质上，显著富含淀粉和蔗糖代谢途径，提示N-糖基化通过调节碳水化合物代谢参与胚胎萌发。值得注意的是，蛋白质-蛋白质相互作用分析揭示了几个油菜素内酯信号蛋白的网络，包括肖恩和其他BR反应蛋白，这意味着糖基化介导的油菜素内酯信号转导可能是调控水稻胚胎萌发的关键机制。综上所述，本研究扩大了我们对水稻蛋白质糖基化的认识，并为水稻种子萌发中的PTM调控提供了新的视角。
Germination is a key event in the angiosperm life cycle. N-glycosylation of proteins is one of the most common post-translational modifications, and has been recognized to be an important regulator of the proteome of the germinating embryo. Here, we report the first N-linked glycosites mapping of rice embryos during germination by using a hydrophilic interaction chromatography (HILIC) glycopeptides enrichment strategy associated with high accuracy mass spectrometry identification. A total of 242 glycosites from 191 unique proteins was discovered. Inspection of the motifs and sequence structures involved suggested that all the glycosites were concentrated within [NxS/T] motif, while 82.3% of them were in a coil structure. N-glycosylation preferentially occurred on proteins with glycoside hydrolase activities, which were significantly enriched in the starch and sucrose metabolism pathway, suggesting that N-glycosylation is involved in embryo germination by regulating carbohydrate metabolism. Notably, protein-protein interaction analysis revealed a network with several Brassinosteroids signaling proteins, including XIAO and other BR-responsive proteins, implying that glycosylation-mediated Brassinosteroids signaling may be a key mechanism regulating rice embryo germination. In summary, this study expanded our knowledge of protein glycosylation in rice, and provided novel insight into the PTM regulation in rice seed germination.