Sequence divergence of the murB and rrfB genes from Escherichia coli and Salmonella typhimurium.
Sequence divergence of the murB and rrfB genes from Escherichia coli and Salmonella typhimurium.
复制标题
大肠杆菌和鼠伤寒沙门氏菌的 murB 和 rrfB 基因的序列差异。
DOI：
10.1007/bf00307771
复制
发表时间：
1994
影响因子：
2.8
通讯作者：
Young,KD
中科院分区：
文献类型：
--
作者：
Dombrosky,PM;Schmid,MB;Young,KD
ThemurBgene ofSalmonella typhimuriumwas cloned and found to be 75% and 82% identical to the DNA and protein sequences, respectively, of the same gene inEscherichia coli. These identities are among the lowest recorded between the two bacteria. Nevertheless, wild-typeS. typhimurium murBcomplemented the known temperature-sensitiveE. colimutant, and wild-typeE. coli murBcomplemented three temperature-sensitive mutants ofS. typhimurium. The 5S rRNA gene,rrfB, and the region betweenmurBandrrfBwere also cloned and sequenced. TherrfBgene ofS. typhimuriumdiffers fromrrfBofE. coliin only 2 of 120 nt, but the region betweenmurBandrrfBhas diverged greatly and includes a sequence that elosely resembles a repetitive extragenic palindrome of the type normally associated withE. coli. Previous comparisons of gene divergence have suggested that the chromosomal mutation rate is lower in the vicinity of the origin of replication. However, theS. typhimurium murBgene, located 6 map minutes from the origin of replication, is highly substituted at synonymous sites and the sequence betweenmurBandrrfBis significantly modified as well. Thus,murBis an exception to the general observation that genes near the origin of replication show less divergence than do genes elsewhere in the bacterial chromosome.
