The stromal-vascular fraction of adipose tissue contributes to major differences between subcutaneous and visceral fat depots
The stromal-vascular fraction of adipose tissue contributes to major differences between subcutaneous and visceral fat depots
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DOI：
10.1002/pmic.201000350
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发表时间：
2010-09-01
期刊：
影响因子：
3.4
通讯作者：
Malagon, Maria A. M.
中科院分区：
文献类型：
--
作者：
Peinado, Juan R.;Jimenez-Gomez, Yolanda;Malagon, Maria A. M.
脂肪组织在细胞组成方面代表了一种复杂的组织，因为它包括成熟的脂肪细胞和构成基质血管部分（SVF）的各种细胞类型，并且根据其解剖位置而具有独特的生化、形态和功能特征。在此，我们对来自人内脏脂肪组织（VAT）和皮下脂肪组织（SAT）脂肪库的成熟脂肪细胞和 SVF 的蛋白质组谱进行了表征，以揭示蛋白质表达的差异，这可能是 VAT 和 SAT 与几种病理的明显关联的基础。具体而言，在瘦个体的 SAT SVF 与 VAT SVF 之间观察到 24 种蛋白质存在差异表达。免疫印迹和 RT-PCR 分析证实了两个脂肪库中核膜蛋白核纤层蛋白 A/C、膜-细胞骨架连接物埃兹蛋白和参与视黄酸生产的酶醛脱氢酶 1A2 的差异调节。总之，观察到具有重要细胞功能的蛋白质在 VAT 和 SAT 之间分布存在差异，并且它们作为 SVF 或成熟脂肪细胞成分的表征为未来研究各种脂肪组织相关病理（如代谢综合征或脂肪营养不良）的分子基础铺平了道路。
Adipose tissue represents a complex tissue both in terms of its cellular composition, as it includes mature adipocytes and the various cell types comprising the stromal-vascular fraction (SVF), and in relation to the distinct biochemical, morphological and functional characteristics according to its anatomical location. Herein, we have characterized the proteomic profile of both mature adipocyte and SVF from human visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) fat depots in order to unveil differences in the expression of proteins which may underlie the distinct association of VAT and SAT to several pathologies. Specifically, 24 proteins were observed to be differentially expressed between SAT SVF versus VAT SVF from lean individuals. Immunoblotting and RT-PCR analysis confirmed the differential regulation of the nuclear envelope proteins lamin A/C, the membrane-cytoskeletal linker ezrin and the enzyme involved in retinoic acid production, aldehyde dehydrogenase 1A2, in the two fat depots. In sum, the observation that proteins with important cell functions are differentially distributed between VAT and SAT and their characterization as components of SVF or mature adipocytes pave the way for future research on the molecular basis underlying diverse adipose tissue-related pathologies such as metabolic syndrome or lipodystrophy.