Development and characterization of antibody reagents for detecting nanoparticles.
Development and characterization of antibody reagents for detecting nanoparticles.
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用于检测纳米颗粒的抗体试剂的开发和表征。
DOI：
10.1039/c5nr04882f
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发表时间：
2015-12-21
期刊：
影响因子：
6.7
通讯作者：
DeLouise LA
中科院分区：
文献类型：
--
作者：
Ravichandran S;Sullivan MA;Callahan LM;Bentley KL;DeLouise LA
纳米颗粒(NPs)在技术应用和商业产品中的使用越来越多，加剧了对环境健康和安全的担忧。环境和生物系统中NPs的检测受到与常用分析技术相关的限制的挑战。在这篇文章中，我们报告了NP结合抗体的发展和特征，称为NProbe。用噬菌体展示方法发现与分散在溶液中的NPs结合的抗体。我们提出了一种产生NProbe的概念验证，并将其用于在体外和体外人类皮肤模型中检测量子点和二氧化钛纳米粒子。用于检测成分、形状、大小和表面涂层不同的NPs的NProbe的持续开发和改进将构成一个强大的工具包，可用于推动纳米技术研究，特别是在纳米毒理学和纳米治疗领域。
The increasing use of nanoparticles (NPs) in technological applications and in commercial products has escalated environmental health and safety concerns. The detection of NPs in the environment and in biological systems is challenged by limitations associated with commonly used analytical techniques. In this paper we report on the development and characterization of NP binding antibodies, termed NProbes. Phage display methodology was used to discover antibodies that bind NPs dispersed in solution. We present a proof-of-concept for the generation of NProbes and their use for detecting quantum dots and titanium dioxide NPs in vitro and in an ex vivo human skin model. Continued development and refinement of NProbes to detect NPs that vary in composition, shape, size, and surface coating will comprise a powerful tool kit that can be used to advance nanotechnology research particularly in the nanotoxicology and nanotherapeutics fields.