INVITRO CATENATION AND DECATENATION OF DNA AND A NOVEL EUKARYOTIC ATP-DEPENDENT TOPOISOMERASE
INVITRO CATENATION AND DECATENATION OF DNA AND A NOVEL EUKARYOTIC ATP-DEPENDENT TOPOISOMERASE
复制标题
DOI：
10.1016/0092-8674(80)90632-7
复制
发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
TOCCHINIVALENTINI, GP
中科院分区：
文献类型：
--
作者：
BALDI, MI;BENEDETTI, P;TOCCHINIVALENTINI, GP
非洲爪蟾生发囊泡的提取物使双链 DNA 环互锁形成索烷。连锁反应需要 Mg2+ 和 ATP。负超螺旋或松弛的 DNA 可用作串联反应的底物。供体和受体 DNA 之间不需要同源性，因为链烷是在具有不相关序列的 DNA 分子之间形成的。在分离负责连锁反应的活性的过程中，发现了一种新的 ATP 依赖性拓扑异构酶。含有新型拓扑异构酶链酸和癸酸 DNA 的级分，离子强度决定将发生 2 个相反反应中的哪一个。
Extracts from Xenopus laevis germinal vesicles interlock duplex DNA circles to form catenanes. The catenation activity requires Mg2+ and ATP. Negatively supercoiled or relaxed DNA can be used as substrates for the catenation reaction. Homology between donor and acceptor DNA is not required, since catenanes are formed between DNA molecules with unrelated sequences. In the course of the isolation of the activity responsible for the catenation reaction, a new ATP-dependent topoisomerase was discovered. The fractions containing the novel topoisomerase catenate and decatenate DNA, the ionic strength dictating which of the 2 opposing reactions will occur.