Analysis of natural allelic variation in Arabidopsis using a multiparent recombinant inbred line population
Analysis of natural allelic variation in Arabidopsis using a multiparent recombinant inbred line population
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DOI：
10.1073/pnas.1100465108
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发表时间：
2011-03-15
影响因子：
11.1
通讯作者：
van Eeuwijk, Fred A.
中科院分区：
文献类型：
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作者：
Huang, Xueqing;Paulo, Maria-Joao;van Eeuwijk, Fred A.
为了利用拟南芥的多样性，将八个创始种质杂交产生六个重组自交系 (RIL) 亚群，统称为拟南芥多亲本 RIL (AMPRIL) 群体。创始人两两杂交产生了四个 F1 杂种。这些F1根据双列杂交方案进行杂交。然后将所得的后代自交三代。使用 SNP 和微卫星标记对 F4 代进行基因分型。开花时间和叶子形态特征的数据在F5代中确定。使用专门开发的混合模型方法对这些性状进行数量性状位点（QTL）分析，从而可以测试 QTL 主效应、背景相互作用的 QTL 以及 QTL 相互作用的 QTL。由于 RIL 在 F4 代中进行基因分型并在 F5 代中进行表型分析，因此残余杂合性可用于确认和精细定位包含此类杂合性的品系的自交后代中的许多 QTL。 AMPRIL 群体是研究复杂性状的一个有吸引力的资源。
To exploit the diversity in Arabidopsis thaliana, eight founder accessions were crossed to produce six recombinant inbred line (RIL) subpopulations, together called an Arabidopsis multiparent RIL (AMPRIL) population. Founders were crossed pairwise to produce four F1 hybrids. These F1s were crossed according to a diallel scheme. The resulting offspring was then selfed for three generations. The F4 generation was genotyped with SNP and microsatellite markers. Data for flowering time and leaf morphology traits were determined in the F5 generation. Quantitative trait locus (QTL) analysis for these traits was performed using especially developed mixed-model methodology, allowing tests for QTL main effects, QTL by background interactions, and QTL by QTL interactions. Because RILs were genotyped in the F4 generation and phenotyped in the F5 generation, residual heterozygosity could be used to confirm and fine-map a number of the QTLs in the selfed progeny of lines containing such heterozygosity. The AMPRIL population is an attractive resource for the study of complex traits.