Changes of higher order chromatin arrangements during major genome activation in bovine preimplantation embryos
Changes of higher order chromatin arrangements during major genome activation in bovine preimplantation embryos
复制标题
DOI：
10.1016/j.yexcr.2009.02.016
复制
发表时间：
2009-07-15
影响因子：
3.7
通讯作者：
Brero, Alessandro
中科院分区：
文献类型：
--
作者：
Koehler, Daniela;Zakhartchenko, Valeri;Brero, Alessandro
基因密集的染色体区域(CT)通常位于哺乳动物细胞核中更内部、更外围的缺乏基因的CT中。在这里，我们证明了这种基因密度相关的CT定位分别适用于牛成纤维细胞和淋巴细胞核中基因最丰富和最缺乏基因的牛染色体19和20。为了确定在发育过程中这种非随机CT序列的建立时间，我们对体外受精产生的三维保存的牛着床前胚胎进行了荧光原位杂交，并调查了BTA 19和20个CT的分布。直到8细胞期，CT19和CT20的径向排列是相同的。然而，在10-16细胞阶段，CT19更多地位于内部，而CT20更多地位于外围，这一显著差异变得明显。由于牛胚胎的主要基因组激活发生在8-16-细胞阶段，我们的发现表明转录激活与卵裂球细胞核染色质拓扑的主要重排之间存在时间相关性。(C)2009 Elsevier Inc.保留所有权利。
Gene-dense chromosome territories (CTs) are typically located more interior, gene-poor CTs more peripheral in mammalian cell nuclei. Here, we show that this gene-density correlated CT positioning holds for the most gene-rich and gene-poor bovine chromosomes 19 and 20, respectively, in bovine fibroblast and lymphocyte nuclei. In order to determine the period at which this non-random CT order is established during development, we performed fluorescence in Situ hybridization, on three-dimensionally preserved bovine preimplantation embryos generated by in vitro fertilization and investigated the distribution of BTA 19 and 20 CTs. Radial arrangements of CTs 19 and 20 were the same up to the 8-cell stage. At the 10- to 16-cell stage, however, a significant difference became apparent with CTs 19 localized more internally and CTs 20 more peripherally. Since major genome activation in bovine embryos occurs at the 8- to 16-cell stage, our findings demonstrate a temporal correlation between transcriptional activation and a major rearrangement of chromatin topography in blastomere nuclei. (C) 2009 Elsevier Inc. All rights reserved.