De novo generation of defective interfering-like RNAs in broad bean mottle bromovirus.
De novo generation of defective interfering-like RNAs in broad bean mottle bromovirus.
复制标题
蚕豆斑驳溴病毒中缺陷型干扰样 RNA 的从头生成。
DOI：
10.1006/viro.1995.1515
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Bujarski,JJ
中科院分区：
文献类型：
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作者：
Pogany,J;Romero,J;Huang,Q;Sgro,JY;Shang,H;Bujarski,JJ
蚕豆斑驳病毒（BBMV）是已知积累缺陷干扰（DI）RNA的溴病毒的唯一成员（Romero et al.，Virology 194，576-584，1993）。在BBMV在蚕豆中连续传代期间，使用无DI RNA的病毒体RNA制备物或转录的基因组RNA接种物证明了DI样RNA的从头产生。对于先前描述的DI RNA，除了一个之外，所有表征的从头产生的DI样RNA都是通过从RNA 2组分中的单个框内缺失衍生的。唯一的例外是来自RNA 2的两个较短的框内缺失。所有DI-样RNA的开放阅读框的维持表明编码能力和/或缩短的2a蛋白在感染期间这些RNA的积累中的重要性。缺失连接位点位于核苷酸1152和2366之间，表明保留的区域对于BBMV DI样RNA在植物中的有效积累是必需的。在5′和3′交界处显示出序列相似性和/或互补性的短区域。我们推测这些区域可以促进DI（DI样）RNA的形成。除了DI-样RNA，BBMV的类型和摩洛哥株系的RNA 2组分的全长核苷酸序列被呈现。
Broad bean mottle virus (BBMV) is the only member of the bromoviruses that is known to accumulate defective-interfering (DI) RNAs (Romero et al., Virology 194, 576-584, 1993). De novo generation of DI-like RNAs was demonstrated during serial passages of BBMV in broad bean using either DI RNA-free virion RNA preparations or transcribed genomic RNA inocula. As for previously described DI RNAs, all but one of the characterized de novo generated DI-like RNAs were derived by a single in-frame deletion from the RNA2 component. The sole exception was derived by two shorter in-frame deletions from RNA2. The maintenance of an open reading frame by all DI-like RNAs suggests the importance of coding capacity and/or the shortened 2a protein in the accumulation of these RNAs during infection. The deletion junction sites were between nucleotides 1152 and 2366, suggesting that the retained regions are essential for the efficient accumulation of BBMV DI-like RNAs in planta. Short regions of sequence similarity and/or complementarity were revealed at the 5′ and 3′ junction borders. We speculate that these regions can facilitate DI (DI-like) RNA formation. In addition to DI-like RNAs, the full-length nucleotide sequences of RNA2 components of the Type and Morocco strains of BBMV are presented.