Surface-Bubble-Modulated Liquid Chromatography: A New Approach for Manipulation of Chromatographic Retention and Investigation of Solute Distribution at Water/Hydrophobic Interfaces
Surface-Bubble-Modulated Liquid Chromatography: A New Approach for Manipulation of Chromatographic Retention and Investigation of Solute Distribution at Water/Hydrophobic Interfaces
复制标题
DOI：
10.1021/ac503802q
复制
发表时间：
2015-01-20
影响因子：
7.4
通讯作者：
Shibukawa, Masami
中科院分区：
文献类型：
--
作者：
Nakamura, Keisuke;Nakamura, Hiroki;Shibukawa, Masami
在本文中，我们提出了一种新的色谱方法，称为表面气泡调制液相色谱（SBMLC），具有混合分离介质与表面纳米气泡。通过将水输送到填充有纳米多孔材料的干燥柱中，可以将纳米气泡或纳米级气相固定在水和疏水材料之间的界面处。气相在疏水表面的掺入导致由气相、疏水部分和水/疏水界面或界面水组成的混合分离系统的形成。可以通过施加到柱上的压力来改变气相的体积，这又改变了水/疏水界面的面积或界面水的体积，而疏水部分的量保持恒定。因此，这一策略提供了一种新的技术，不仅用于操纵分离选择性的压力，但也阐明了在水溶液中的溶质化合物的积累或保留的疏水材料的机制。我们评估的贡献的界面水在十八烷基键合二氧化硅的表面和键合层本身的保留各种溶质化合物在水溶液中的柱上填充的材料由SBMLC。结果表明，在疏水表面形成的界面水虽然体积很小，但对截留起着关键作用。实验证明了压力对SBMLC分离选择性的影响。
In this paper, we present a new chromatographic method termed surface-bubble-modulated liquid chromatography (SBMLC), that has a hybrid separation medium incorporated with surface nanobubbles. Nanobubbles or nanoscale gas phases can be fixed at the interface between water and a hydrophobic material by delivering water into a dry column packed with a nanoporous material. The incorporation of a gas phase at the hydrophobic surface leads to the formation of the hybrid separation system consisting of the gas phase, hydrophobic moieties, and the water/hydrophobic interface or the interfacial water. One can change the volume of the gas phase by pressure applied to the column, which in turn alters the area of water/hydrophobic interface or the volume of the interfacial water, while the amount of the hydrophobic moiety remains constant. Therefore, this strategy provides a novel technique not only for manipulating the separation selectivity by pressure but also for elucidating the mechanism of accumulation or retention of solute compounds in aqueous solutions by a hydrophobic material. We evaluate the contributions of the interfacial water at the surface of an octadecyl bonded silica and the bonded layer itself to the retention of various solute compounds in aqueous solutions on the column packed with the material by SBMLC. The results show that the interfacial water formed at the hydrophobic surface has a key role in retention even though its volume is rather small. The manipulation of the separation selectivity of SBMLC for some organic compounds by pressure is demonstrated.