Identification of six putative human transporters with structural similarity to the drug transporter SLC22 family
Identification of six putative human transporters with structural similarity to the drug transporter SLC22 family
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DOI：
10.1016/j.ygeno.2007.03.017
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发表时间：
2007-11-01
期刊：
影响因子：
4.4
通讯作者：
Fredriksson, Robert
中科院分区：
文献类型：
--
作者：
Jacobsson, Josefin A.;Haitina, Tatjana;Fredriksson, Robert
溶质载体家族22(SLC22)是一个有机阳离子和阴离子转运蛋白大家族。这些是主要在肾脏和肝脏表达的跨膜蛋白，不能从体内摄取和排泄环境毒素、内源性物质和药物。通过全面的数据库搜索，我们鉴定了六个尚未在参考序列数据库中克隆或注释的人类蛋白质。其中5个属于SLC22家族，分别是SLC22A20、SLC22A23、SLC22A24、SLC22A25和SPNS3，第六个基因SVOPL与突触小泡蛋白SVOP同源。我们确定了这些基因在小鼠和大鼠中的同源基因以及其他同源蛋白，并首次对人、小鼠和大鼠的整个SLC22家族进行了系统发育分析。此外，我们还进行了系统发育分析，结果表明，与所有脊椎动物蛋白相比，SVOP和SV2A-C与SLC22家族最为相似。最后，我们使用实时定量聚合酶链式反应对30个大鼠组织中的15个基因进行了组织定位研究。(C)2007 Elsevier Inc.保留所有权利。
The solute carrier family 22 (SLC22) is a large family of organic cation and anion transporters. These are transmembrane proteins expressed predominantly in kidneys and liver and inediate the uptake and excretion of environmental toxins, endogenous substances, and drugs from the body. Through a comprehensive database search we identified six human proteins not yet cloned or annotated in the reference sequence databases. Five of these belong to the SLC22 family, SLC22A20, SLC22A23, SLC22A24, SLC22A25, and SPNS3, and the sixth gene, SVOPL, is a paralog to the synaptic vesicle protein SVOP. We identified the orthologs for these genes in mouse and rat and additional homologous proteins and performed the first phylogenetic analysis on the entire SLC22 family in human, mouse, and rat. In addition, we performed a phylogenetic analysis which showed that SVOP and SV2A-C are, in a comparison with all vertebrate proteins, most similar to the SLC22 family. Finally, we performed a tissue localization study on 15 genes on a panel of 30 rat tissues using quantitative real-time polymerase chain reaction. (c) 2007 Elsevier Inc. All rights reserved.