Gtsf1 is essential for proper female sex determination and transposon silencing in the silkworm, Bombyx mori.
Gtsf1 is essential for proper female sex determination and transposon silencing in the silkworm, Bombyx mori.
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Gtsf1 对于家蚕 (Bombyx mori) 中正确的雌性性别决定和转座子沉默至关重要
DOI：
10.1371/journal.pgen.1009194
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发表时间：
2020-11
期刊：
影响因子：
4.5
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Chen K;Yu Y;Yang D;Yang X;Tang L;Liu Y;Luo X;Walters JR;Liu Z;Xu J;Huang Y
昆虫的性别决定途径是惊人的多样性。例如，家蚕独特地使用皮尔纳途径的各种组分来产生Fem信号以指定雌性命运。在这项研究中，我们鉴定了BmGTSF 1作为参与B的新型皮尔纳因子。森性决定我们发现，BmGtsf 1有一个独特的表达模式相比，果蝇和小鼠。CRISPR/Cas9诱导的BmGtsf 1突变通过将下游靶标BmMasc和Bmdsx的表达转变为雄性模式，导致基因型雌性动物的部分性逆转。由于Fem piRNA的水平在雌性突变体中显著降低，我们得出结论，BmGtsf 1在雌性化信号的生物发生中起关键作用。我们还表明，BmGTSF 1物理相互作用与BmSIWI，以前报道的蛋白质参与女性性别决定，表明BmGTSF 1功能的BmSIWI的辅因子。BmGtsf 1突变导致皮尔纳途径失调，包括皮尔纳生物合成缺陷和转座子去阻遏，表明BmGtsf 1也是家蚕中的皮尔纳因子。此外，我们发现，BmGtsf 1突变导致配子发生缺陷的男性和女性。我们的数据表明，BmGtsf 1是一个新的成分参与性别决定途径在B。森。
Sex determination pathways are astoundingly diverse in insects. For instance, the silk moth Bombyx mori uniquely use various components of the piRNA pathway to produce the Fem signal for specification of the female fate. In this study, we identified BmGTSF1 as a novel piRNA factor which participates in B. mori sex determination. We found that BmGtsf1 has a distinct expression pattern compared to Drosophila and mouse. CRISPR/Cas9 induced mutation in BmGtsf1 resulted in partial sex reversal in genotypically female animals by shifting expression of the downstream targets BmMasc and Bmdsx to the male pattern. As levels of Fem piRNAs were substantially reduced in female mutants, we concluded that BmGtsf1 plays a critical role in the biogenesis of the feminizing signal. We also demonstrated that BmGTSF1 physically interacted with BmSIWI, a protein previously reported to be involved in female sex determination, indicating BmGTSF1 function as the cofactor of BmSIWI. BmGtsf1 mutation resulted in piRNA pathway dysregulation, including piRNA biogenesis defects and transposon derepression, suggesting BmGtsf1 is also a piRNA factor in the silkworm. Furthermore, we found that BmGtsf1 mutation leads to gametogenesis defects in both male and female. Our data suggested that BmGtsf1 is a new component involved in the sex determination pathway in B. mori.
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影响因子：
16.6
作者：
Ding D;Liu J;Dong K;Midic U;Hess RA;Xie H;Demireva EY;Chen C
通讯作者：
Chen C
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
16
作者：
Ge, Daniel Tianfang;Wang, Wei;Zamore, Phillip D.
通讯作者：
Zamore, Phillip D.
影响因子：
13.8
作者：
Czech B;Hannon GJ
通讯作者：
Hannon GJ
DOI：
10.1073/pnas.0805993105
发表时间：
2008-11-25
影响因子：
11.1
作者：
Gonzalez, Alejandra N.;Lu, Hong;Erickson, James W.
通讯作者：
Erickson, James W.