XANTHINE DEHYDROGENASE ACCUMULATION IN DEVELOPING DROSOPHILA EYES
XANTHINE DEHYDROGENASE ACCUMULATION IN DEVELOPING DROSOPHILA EYES
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DOI：
10.1016/0022-1910(75)90206-1
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发表时间：
1975-01-01
影响因子：
2.2
通讯作者：
DAVIDSON, NA
中科院分区：
文献类型：
--
作者：
BARRETT, D;DAVIDSON, NA
黄嘌呤脱氢酶活性通过荧光分光光度法在反应的波长峰处跟踪蝶呤氧化成异黄蝶呤来测定：激发，344 nm;发射，412 nm。该方法对小于0.1 μU的活性（0.1 pmol/min）敏感，并允许测定果蝇眼盘匀浆。当幼虫眼盘含有小于个体XDH的0.1%时，发育中的眼睛成为一个主要的储存库，到眼睛色素沉着时，个体活性的30%在眼睛中。这些数据表明了一个众所周知的非自主行动的generosy的基础，XDH的结构基因：该酶是在一个器官的主要基因表达合成，并通过血淋巴运输到眼睛的蛹和pharate成人。
Xanthine dehydrogenase activity is assayed by following the oxidation of pterin to isoxanthopterin by spectrofluorometry at the reaction's wavelength peaks: excitation, 344 nm; emission, 412 nm. The method is sensitive to less than 0·1 μU of activity (0·1 pmol/min) and allows the assay ofDrosophilaimaginal disk homogenates.While the larval eye disk contains less than 0·1 per cent of the individual's XDH, the developing eye becomes a major store, with 30 per cent of the individual's activity by the time of eye pigmentation. The data suggest a basis for the well-known non-autonomous action of the generosy, the structural gene for XDH: the enzyme is synthesized in an organ of primary gene expression, and transported through the haemolymph to the eye of the pupa and pharate adult.