Molecular genetic study of the gutter shaped root (GSR) on mouse chromosome 17.
Molecular genetic study of the gutter shaped root (GSR) on mouse chromosome 17.
复制标题
小鼠 17 号染色体上的沟状根 (GSR) 的分子遗传学研究。
作者：
K. Matsune
在这项研究中，一个遗传因素影响的外观沟形根（GSR）的小鼠使用两个近交系，C57 L/J和C57 BL/6 J，以及这些品系的遗传杂交。利用小鼠第17号染色体上的120个Mit标记筛选出两品系间的多态性标记。利用聚合酶链反应（PCR）技术获得了9个在两株间具有多态性的Mit标记。采用简单序列长度A多态性（SSLP）分析方法，对66只回交小鼠进行个体基因型检测。从使用9个信息标记的个体基因分型的结果，确定最高连锁在D17 Mit 260。由此推测，引起小鼠GSR的基因可能位于小鼠17号染色体D17 Mit 260附近。
In this study, a genetic factor affecting the appearance of the gutter-shaped root (GSR) in mice was examined using two inbred strains of mice, C57L/J and C57BL/6J, and the genetic crosses of these strains. 120 Mit markers, which exist on mouse chromosome 17, have been investigated to select polymorphic markers between the two strains. Nine Mit markers with polymorphism between the two strains were obtained using polymerase chain reaction (PCR). To detect the individual genotype of 66 backcross mice, simple sequence length A polymorphism (SSLP) analysis was performed. From the results of individual genotyping using 9 informative markers, the highest linkage was determined at D17Mit260. Based on these findings, it was suggested that the gene causing the mouse GSR might be located on mouse chromosome 17 and nearby D17Mit260.
DOI：
10.1006/meth.1997.0576
发表时间：
1998-02-01
期刊：
METHODS-A COMPANION TO METHODS IN ENZYMOLOGY
影响因子：
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作者：
Eppig, JT;Blake, JA;Richardson, JE
通讯作者：
Richardson, JE