Sex-specific histone modifications in mouse fetal and neonatal germ cells
Sex-specific histone modifications in mouse fetal and neonatal germ cells
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DOI：
10.2217/epi-2018-0193
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发表时间：
2019-04-01
期刊：
影响因子：
3.8
通讯作者：
Kono, Tomohiro
中科院分区：
文献类型：
--
作者：
Kawabata, Yukiko;Kamio, Asuka;Kono, Tomohiro
目的：生殖细胞的表观遗传特征被动态重编程以诱导适当的分化、发育和性别特化。我们研究了小鼠胎儿生殖细胞（FGC）和新生儿生殖细胞的性别特异性表观遗传变化。材料与方法：采用染色质免疫沉淀和测序技术，对小鼠E13.5胎精细胞和P1胎精细胞中的6个组蛋白标记进行分析。将这些数据集与转座酶可接近的染色质位点、DNA甲基化和转录组进行比较。结果如下：H3 K4 me 3/H3 K27 me 3双价标记在雌雄FGC的不同染色体区域富集。结论：我们的研究结果表明，组蛋白修饰可能会影响FGC基因的表达后，DNA甲基化擦除，有助于分化为女性和男性生殖细胞。
Aims: Epigenetic signatures of germline cells are dynamically reprogrammed to induce appropriate differentiation, development and sex specification. We investigated sex-specific epigenetic changes in mouse fetal germ cells (FGCs) and neonatal germ cells. Materials & methods: Six histone marks in mouse E13.5 FGCs and P1 neonatal germ cells were analyzed by chromatin immunoprecipitation and sequencing. These datasets were compared with transposase-accessible chromatin sites, DNA methylation and transcriptome. Results: Different patterns of each histone mark were detected in female and male FGCs, and H3K4me3/H3K27me3 bivalent marks were enriched in different chromosomal regions of female and male FGCs. Conclusion: Our results suggest that histone modifications may affect FGC gene expression following DNA methylation erasure, contributing to the differentiation into female and male germ cells.