Bio-orthogonal Fluorescent Labelling of Biopolymers through Inverse-Electron-Demand Diels-Alder Reactions.
Bio-orthogonal Fluorescent Labelling of Biopolymers through Inverse-Electron-Demand Diels-Alder Reactions.
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DOI：
10.1002/cbic.201600607
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发表时间：
2017-03-16
期刊：
影响因子：
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通讯作者：
Kele P
中科院分区：
文献类型：
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作者：
Kozma E;Demeter O;Kele P
基于反电子需求狄尔斯-阿尔德（IEDDA）环加成的生物正交标记方案最近引起了化学生物学的广泛关注。该反应具有快速反应动力学、完全生物正交性和高选择性等吸引人的特点，帮助化学生物学家在分子水平上更深入地了解生化过程。我们列出了这些试剂的成分并讨论了这些试剂的可能性和局限性，提供了基于 IEDDA 的生物分子操作领域的最新快照，特别关注通过使用生物正交构建块的荧光调制方法。最后，我们讨论了进一步发展需要解决的挑战，以克服最近的限制并使研究人员能够更详细地回答生物分子问题。
Bio‐orthogonal labelling schemes based on inverse‐electron‐demand Diels–Alder (IEDDA) cycloaddition have attracted much attention in chemical biology recently. The appealing features of this reaction, such as the fast reaction kinetics, fully bio‐orthogonal nature and high selectivity, have helped chemical biologists gain deeper understanding of biochemical processes at the molecular level. Listing the components and discussing the possibilities and limitations of these reagents, we provide a recent snapshot of the field of IEDDA‐based biomolecular manipulation with special focus on fluorescent modulation approaches through the use of bio‐orthogonalized building blocks. At the end, we discuss challenges that need to be addressed for further developments in order to overcome recent limitations and to enable researchers to answer biomolecular questions in more detail.