Quantitative measurement of spatial effects of DNA origami on molecular binding reactions detected using atomic force microscopy
Quantitative measurement of spatial effects of DNA origami on molecular binding reactions detected using atomic force microscopy
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定量测量 DNA 折纸对原子力显微镜检测的分子结合反应的空间影响
DOI：
10.1021/acsami.9b01691
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发表时间：
2019
影响因子：
9.5
通讯作者：
Li Bin
中科院分区：
文献类型：
--
作者：
Zhang Ping;Wang Fei;Liu Wenjing;Mao Xiuhai;Hao Changchun;Zhang Yi;Fan Chunhai;Hu Jun;Wang Lihua;Li Bin
DNA折纸是一种普遍存在的纳米结构，可以用作构建分子马达、纳米传感器、纳米药物和光学器件的通用支架。了解DNA折纸结构的内在异质性对于优化高效纳米器件的设计至关重要。在这里，我们用原子力显微镜研究了DNA折纸对结合反应的空间效应。蛋白质复合物在DNA折纸的顶点和边缘比在DNA折纸的表面更有效地形成；令人惊讶的是，生物素-链霉亲和素结合效率的最大差异超过80%，结合率的变化约为40倍，这表明在DNA折纸的不同位置存在不同的微环境。我们的发现不仅有助于DNA折纸的潜在应用，而且有助于澄清纳米材料的不均匀分布或缺陷引起的差异。
DNA origami is a ubiquitous nanostructure that can be used as a universal scaffold for constructing molecular motors, nanosensors, nanodrugs, and optical devices. Understanding the inherent heterogeneity of DNA origami structures is crucial for optimizing the design of high-efficiency nanosized-devices. Here, we investigated the spatial effects of the DNA origami on binding reactions using atomic force microscopy. Protein complexes formed more efficiently at the vertex and rim than on the surface of the DNA origami; surprisingly, the maximum difference in biotin–streptavidin binding efficiency was over 80%, and the change in the binding rate was approximately 40-fold, suggesting the presence of distinct microenvironments at different locations of the DNA origami. Our findings are not only useful for the potential applications of the DNA origami, but also for clarifying differences in nanomaterials caused by nonuniform distribution or defects.