Downregulation of Rice DWARF 14 LIKE Suppress Mesocotyl Elongation via a Strigolactone Independent Pathway in the Dark
Downregulation of Rice DWARF 14 LIKE Suppress Mesocotyl Elongation via a Strigolactone Independent Pathway in the Dark
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DOI：
10.1016/j.jgg.2014.12.003
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发表时间：
2015-03-20
影响因子：
5.9
通讯作者：
Kyozuka, Junko
中科院分区：
文献类型：
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作者：
Kameoka, Hiromu;Kyozuka, Junko
独脚金内酯 (SL) 是一类植物激素，可根据环境条件控制植物发育。在水稻中，中胚轴伸长在黑暗中受 SL 调节，而 SL 缺陷或不敏感突变体中中胚轴更长。 SL 由 DWARF14 (D14) 感知，DWARF14 是一个小基因家族的成员。在这项研究中，我们检查了水稻中另一个 D14 家族基因 D14 LIKE (D14L) 的功能，重点关注中胚轴生长。在黑暗中，D14L RNAi 系的中胚轴比 WT 的中胚轴长。当 D14L RNAi 系与 d14 突变结合时，这种表型得到增强，表明 D14 和 D14L 独立发挥作用，抑制中胚轴伸长。这种表型通过外源供应 GR24（一种合成 SL）得到缓解，表明 D14L 对于 SL 信号传导不是必需的。 D14L mRNA 主要在维管束和冠根原基中表达。我们的结果表明，D14L 和 D14 分别通过 SL 独立途径和 SL 信号途径发挥作用。
Strigolactones (SLs) are a class of plant hormones that control plant development in response to environmental conditions. In rice, mesocotyl elongation is regulated by SLs in the dark, while mesocotyls are longer in SL deficient or insensitive mutants. SLs are perceived by DWARF14 (D14), which is a member of a small gene family. In this study, we examined the function of another D14 family gene in rice, D14 LIKE (D14L), focusing on mesocotyl growth. The mesocotyls of D14L RNAi lines are longer than those of WT in the dark. This phenotype is enhanced when the D14L RNAi lines are combined with the d14 mutation, suggesting that D14 and D14L work independently to inhibit mesocotyl elongation. This phenotype is alleviated by the exogenous supply of GR24, a synthetic SL, suggesting that D14L is not necessary for SL signaling. D14L mRNA is predominantly expressed in vascular bundles and crown root primordia. Our results suggest that D14L and D14 confer their effects via an SL independent pathway and an SL signaling pathway respectively.