Suppression of pine wilt disease by an antibacterial agent, oxolinic acid
Suppression of pine wilt disease by an antibacterial agent, oxolinic acid
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DOI：
10.1002/ps.1920
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发表时间：
2010-06-01
影响因子：
4.1
通讯作者：
Kim, Jin-Cheol
中科院分区：
文献类型：
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作者：
Kwon, Hyeok Ran;Choi, Gyung Ja;Kim, Jin-Cheol
背景：松材萎蔫病（PWD）是一种非常复杂的疾病，据报道是由松材线虫Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle及其伴生细菌引起的。但目前尚未见抗菌药物控制PWD的报道。本研究旨在探讨抗菌药物对PWD的控制效果。结果：在所检测的6种抗菌药物中，氧喹啉酸（OA）对3株松材线虫分离的5种细菌的抑菌活性最强。在体内实验中，它有效地抑制了3年树苗PWD的发展。和调查。当每株幼苗注射3mg时，防治效果为71%。OA与杀线虫剂阿维菌素（abamectin, Ab）混合使用对PWD的防治效果优于OA和鲍鱼。此外，在野外条件下，OA单独和OA与Ab的混合也能控制大约20年的松树的PWD。结论：本文首次报道OA对PWD的抑制作用。结果表明，单用抗菌药物或抗菌与杀线虫剂联用均可有效控制PWD。(C) 2010年化学工业学会
BACKGROUND: Pine wilt disease (PWD) is very complex and has been reported to be caused by pine wood nematode, Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle, and its accompanying bacteria. However, there is no report on the control of PWD by antibacterial agent. The present study was performed to investigate disease control efficacy of antibacterial agents against PWD.RESULTS: Among six antibacterial antibiotics tested, oxolinic acid (OA) showed the strongest antibacterial activity against five bacteria isolated from three strains of pine wood nematode. In in vivo assay, it effectively suppressed the development of PWD in three-year-old seedlings of Pinus densiflora Sieb. & Zucc.; it showed 71% control when injected at 3 mg per seedling. A mixture of OA and the nematicidal agent abamectin (Ab) showed higher disease control efficacy against PWD than either OA or Abalone. In addition, OA alone and a mixture of OA and Ab also controlled PWD in approximately 20-year-old pine trees under field conditions.CONCLUSION: This is the first report on the suppression of PWD by OA. The result strongly indicates that PWD could be controlled by antibacterial antibiotic alone and a combination of antibacterial and nematicidal agents. (C) 2010 Society of Chemical Industry