Identification of neutrophils in the nonsensory epithelium of the vomeronasal organ in virus-antibody-free rats.
Identification of neutrophils in the nonsensory epithelium of the vomeronasal organ in virus-antibody-free rats.
复制标题
无病毒抗体大鼠犁鼻器非感觉上皮中中性粒细胞的鉴定。
DOI：
10.1007/bf00304519
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发表时间：
1995
影响因子：
3.6
通讯作者：
Kulkarni,AP
中科院分区：
文献类型：
--
作者：
Getchell,ML;Kulkarni,AP
浸润无病毒抗体大鼠犁鼻器非感觉上皮的细胞表现出对 β2-微球蛋白和免疫球蛋白 (Ig) E 的表面免疫反应性。通过使用针对细胞特异性标记物的抗体的免疫组织化学技术或针对具有表面受体的免疫细胞的组织化学技术来进一步表征它们。 免疫球蛋白E。细胞内颗粒对乳铁蛋白和 CD18（一种白细胞粘附分子）具有免疫反应性，明确地将这些细胞鉴定为中性粒细胞。犁鼻器以及鼻腔其余部分中 IgA 和 IgG 免疫反应性 B 淋巴细胞、T 淋巴细胞和辅助免疫细胞数量较少，证实不存在感染。我们假设犁鼻泵的操作会引起短暂性局灶性缺血的反复发作，然后再灌注，从而导致中性粒细胞趋化剂的释放和粘附因子的调节，从而调节中性粒细胞外渗和迁移到非感觉上皮中。白细胞介素 8 的免疫反应性分布表明它不是该系统中主要的中性粒细胞趋化剂，而 CD18 的免疫反应性分布表明它积极参与中性粒细胞外渗。除了在免疫监视中的作用外，中性粒细胞还可以刺激离子/水分泌到犁鼻腔中，影响调节感觉上皮刺激进入和清除的周围感受器过程。
Cells infiltrating the nonsensory epithelium of the vomeronasal organ of virus-antibody-free rats exhibited surface immunoreactivity forβ2-microglobulin and immunoglobulin (Ig) E. They were further characterized by using immunohistochemical techniques with antibodies to cell-specific markers or histochemical techniques for immunocytes with surface receptors for IgE. Localization of intracellular granules immunoreactive for lactoferrin and CD18, a leukocyte adhesion molecule, unequivocally identified these cells as neutrophils. The low number of IgA-and IgG-immunoreactive B lymphocytes, T lymphocytes, and accessory immunocytes in the vomeronasal organ as well as the rest of the nasal cavity confirmed the absence of infection. We hypothesize that the operation of the vomeronasal pump induces repeated episodes of transient focal ischemia followed by reperfusion, which results in release of neutrophil chemoattractants and modulation of adhesion factors that regulate the extravasation and migration of neutrophils into the nonsensory epithelium. The distribution of immunoreactivity for interleukin 8 suggests that it is not the primary neutrophil chemoattractant in this system while that of CD18 suggests its active involvement in neutrophil extravasation. In addition to their role in immune surveillance, neutrophils may stimulate ion/water secretion into the vomeronasal lumen, affecting the perireceptor processes regulating stimulus access and clearance from the sensory epithelium.