Femtosecond Laser Lenticule Transplantation in Rabbit Cornea: Experimental Study
Femtosecond Laser Lenticule Transplantation in Rabbit Cornea: Experimental Study
复制标题
飞秒激光微透镜移植兔角膜的实验研究
DOI：
10.3928/1081597x-20121115-05
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发表时间：
2012-12-01
影响因子：
2.4
通讯作者：
Zhou, XingTao
中科院分区：
文献类型：
--
作者：
Liu, HuiYing;Zhu, WenQing;Zhou, XingTao
目得：目的：探讨飞秒激光诱导兔角膜小切口小梁移植的可行性，并观察移植后角膜组织和神经修复的相关组织学特征。方法：8只健康纯种新西兰白色兔行右眼飞秒激光小切口小梁切除术（SMILE）。将晶状体植入左眼飞秒激光创建的角膜基质袋中，这被定义为飞秒激光角膜晶状体移植。结果：术后早期角膜炎症明显，小眼周组织水肿，细胞活化。组织水肿在术后第10天仍然存在。到1个月时，水肿消退，活化细胞逐渐静止，观察到神经纤维再生。3个月时，植入物与受体角膜融合，细胞外基质逐渐清除，神经纤维变粗。术后6个月，角膜基质纤维的形态和分布接近正常，神经纤维的数量减少.CONCLUSIONS：飞秒激光角膜移植术在兔是可行的，作为微囊被证明茁壮成长，并与受体基质整合。1个月后开始神经再生。[J Refract Surg.2012;28（12）：907-911.]电话：021 - 8888888传真：021 - 88888888
PURPOSE: To evaluate the feasibility of femtosecond laser-induced lenticule transplantation in the rabbit cornea and to observe the relative histologic characteristics of corneal tissue and nerve repair after transplantation.METHODS: Eight healthy, purebred, New Zealand white rabbits underwent femtosecond laser small-incision lenticule extraction (SMILE) surgery in the right eye. Lenticules were inserted into a femtosecond laser-created corneal stromal pocket in the left eye, which was defined as femtosecond laser corneal lenticule transplantation. Postoperative observation and examination were completed to evaluate the surgery.RESULTS: In the early postoperative period, inflammation of the cornea was noted, tissue around the lenticule was edematous, and cells were activated. Tissue edema remained at postoperative day 10. By 1 month, edema had resolved, activated cells gradually became quiescent, and nerve fiber regeneration was observed. By 3 months, the lenticule integrated into the recipient cornea, extracellular matrix gradually cleared, and thicker nerve fibers were noted. By 6 months postoperative, morphology and distribution of the corneal stromal fibers were close to normal, and the number of nerve fibers was reduced.CONCLUSIONS: Femtosecond laser corneal lenticule transplantation in rabbits is feasible, as the lenticule was shown to thrive and integrate with the recipient stroma. Nerve regeneration begins after 1 month. [J Refract Surg. 2012;28(12):907-911.] doi: 10.3928/1081597X-20121115-05