TAKASAGO-6 apparatus for cryogenic coherent X-ray diffraction imaging of biological non-crystalline particles using X-ray free electron laser at SACLA
TAKASAGO-6 apparatus for cryogenic coherent X-ray diffraction imaging of biological non-crystalline particles using X-ray free electron laser at SACLA
复制标题
DOI：
10.1063/1.4948317
复制
发表时间：
2016-06-01
影响因子：
1.6
通讯作者：
Yamamoto, Masaki
中科院分区：
文献类型：
--
作者：
Kobayashi, Amane;Sekiguchi, Yuki;Yamamoto, Masaki
相干x射线衍射成像（CXDI）是一种用于分析微米到亚微米尺度的非晶体颗粒结构的技术。我们开发了一种名为TAKASAGO-6的衍射装置，用于低温下冷冻水合非晶体生物颗粒的单次CXDI实验，该实验使用SPring-8 Angstrom Compact自由电子激光器提供的重复频率为30 Hz的x射线自由电子激光脉冲。样品颗粒被分散在由特殊设计的圆盘支撑的薄膜上后，被快速冷却。该装置配有高速平移台和低温锅，用于以高于25 μ m/33 ms的速度对磁盘进行光栅扫描。此外，我们使用的装置有助于将冷却后的样品从液氮储存器转移到低温锅中。在目前的实验过程中，可以在1小时内收集超过20,000个衍射图案。在这里，我们报告了衍射装置的关键部件和性能。基于衍射数据采集的效率和金属颗粒、生物细胞和细胞器的结构分析，讨论了该衍射仪在生物样品结构分析中的应用前景。AIP出版社出版。
Coherent X-ray diffraction imaging (CXDI) is a technique for structure analyses of non-crystalline particles with dimensions ranging from micrometer to sub-micrometer. We have developed a diffraction apparatus named TAKASAGO-6 for use in single-shot CXDI experiments of frozen-hydrated non-crystalline biological particles at cryogenic temperature with X-ray free electron laser pulses provided at a repetition rate of 30 Hz from the SPring-8 Angstrom Compact free-electron LAser. Specimen particles are flash-cooled after being dispersed on thin membranes supported by specially designed disks. The apparatus is equipped with a high-speed translation stage with a cryogenic pot for raster-scanning of the disks at a speed higher than 25 mu m/33 ms. In addition, we use devices assisting the easy transfer of cooled specimens from liquid-nitrogen storages to the cryogenic pot. In the current experimental procedure, more than 20 000 diffraction patterns can be collected within 1 h. Here we report the key components and performance of the diffraction apparatus. Based on the efficiency of the diffraction data collection and the structure analyses of metal particles, biological cells, and cellular organelles, we discuss the future application of this diffraction apparatus for structure analyses of biological specimens. Published by AIP Publishing.