Motility Resilience of Molecular Shuttles Against Defective Motors
Motility Resilience of Molecular Shuttles Against Defective Motors
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DOI：
10.1109/tnb.2022.3170562
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发表时间：
2022-04
影响因子：
3.9
通讯作者：
Samuel Macharia Kang’iri;T. Nitta
中科院分区：
文献类型：
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作者：
Samuel Macharia Kang’iri;T. Nitta
肌球蛋白和驱动蛋白是在活细胞中发现的生物分子马达。通过推动它们相关的细胞骨架丝，这些生物分子马达促进细胞中的力产生和物质运输。当被提取时，生物分子马达由于其高操作效率和纳米尺寸而成为体外应用例如生物传感器装置的有希望的候选者。然而，在集成到这些设备中的过程中，一些电机由于与基板表面的不利粘附而变得有缺陷。这些有缺陷的马达抑制了细胞骨架细丝的运动性，而细胞骨架细丝构成了装置中使用的分子穿梭机。在实验中控制活跃和有缺陷的马达的比例的困难阻碍了关于分子穿梭运动对有缺陷的马达的阻抗的弹性的系统研究。在这里，我们使用数学模型来系统地检查这些分子穿梭机对有缺陷的电机阻抗的推进弹性。该模型表明，基板上的活性马达的分数是决定的分子穿梭运动的弹性的基本因素。大约40%的主动驱动蛋白或80%的主动肌球蛋白马达需要构成分子穿梭在各自的基板连续滑行。在描述运动行为的数学模型的简单性提供了在阐明分子梭的运动弹性的机制的实用程序。
Myosin and kinesin are biomolecular motors found in living cells. By propelling their associated cytoskeletal filaments, these biomolecular motors facilitate force generation and material transport in the cells. When extracted, the biomolecular motors are promising candidates for in vitro applications such as biosensor devices, on account of their high operating efficiency and nanoscale size. However, during integration into these devices, some of the motors become defective due to unfavorable adhesion to the substrate surface. These defective motors inhibit the motility of the cytoskeletal filaments which make up the molecular shuttles used in the devices. Difficulties in controlling the fraction of active and defective motors in experiments discourage systematic studies concerning the resilience of the molecular shuttle motility against the impedance of defective motors. Here, we used mathematical modelling to systematically examine the resilience of the propulsion by these molecular shuttles against the impedance of the defective motors. The model showed that the fraction of active motors on the substrate is the essential factor determining the resilience of the molecular shuttle motility. Approximately 40% of active kinesin or 80% of active myosin motors are required to constitute continuous gliding of molecular shuttles in their respective substrates. The simplicity of the mathematical model in describing motility behavior offers utility in elucidating the mechanisms of the motility resilience of molecular shuttles.