Dielectrophoresis of DNA: Quantification by impedance measurements
Dielectrophoresis of DNA: Quantification by impedance measurements
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DOI：
10.1063/1.3430550
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发表时间：
2010-06-01
期刊：
影响因子：
3.2
通讯作者：
Hoelzel, Ralph
中科院分区：
文献类型：
--
作者：
Henning, Anja;Bier, Frank. F.;Hoelzel, Ralph
通过测量平面微电极之间的电容变化来确定DNA的介电性质。DNA大小从100个碱基到48个碱基，DNA浓度从小于0.1到70微克/毫升。介电泳谱在3 kHz和3 MHz附近有最大响应。对于很长的DNA片段(大于10kbp)和短的100bp片段，反应最强，这与DNA的持续长度相对应。该方法允许在不需要标记协议或光学可访问性的情况下，简单地、自动地获取亚显微物体的介电性质。(C)2010年美国物理研究所。[DOI：10.1063/1.3430550]
Dielectrophoretic properties of DNA have been determined by measuring capacitance changes between planar microelectrodes. DNA sizes ranged from 100 bp to 48 kbp, DNA concentrations from below 0.1 to 70 mu g/ml. Dielectrophoretic spectra exhibited maximum response around 3 kHz and 3 MHz. The strongest response was found for very long DNA (above 10 kbp) and for short 100 bp fragments, which corresponds to the persistence length of DNA. The method allows for an uncomplicated, automatic acquisition of the dielectrophoretic properties of submicroscopical objects without the need for labeling protocols or optical accessibility. (C) 2010 American Institute of Physics. [doi:10.1063/1.3430550]